[生化] 親和色譜法
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
表達産物用親和層析一步法進行純化，然後用凝膠阻滞試驗觀察重組蛋白結合質粒的能力。
Methods A combination of euglobulin precipitation, ion-exchange chromatography, (NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.
方法采用優球蛋白沉澱、離子交換色譜、（NH4）2SO4沉澱和親和色譜法進行純化。
Polyclonal antibo***s are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human CDK5. Antibo***s are purified by protein A and peptide affinity chromatography.
該多克隆抗體由合成的人類CDK5氨基酸序列對應肽段免疫動物而制成。該抗體使用蛋白A和多肽親和層析純化而得。
Antibo***s are purified by protein A and peptide affinity chromatography.
抗體由蛋白A和肽段親和層析純化得到。
Cu(II)-immobilized metal affinity chromatography (Cu(II)-IMAC), as a highly reliable analytical procedure, has been widely used in separation and purification of biomacromolecule.
銅離子固定金屬親和色譜作為一種有效的分析方法，已普遍應用于生物大分子的分離與純化。
The application of molecular recognition in affinity chromatography, solid extraction, membrane separation technology raises the separation efficiency and separates those optical isomeric compounds.
分子識别應用于親和色譜、固相萃取、膜分離技術極大地促進了分離效率的提高和一些複雜物質的拆分。
Transformants were induced by IPTG, and then expressed. By DTT reduction, the soluble 19 peptide was obtained from chitin affinity chromatography.
表達的融合蛋白經幾丁質親和層析、二硫蘇糖醇（DTT）的柱内還原，直接獲得可溶性19肽。
Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.
而且從周質空間分離的蛋白經過鎳住得到了進一步的純化。
Polyclonal antibo***s are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PRAS40. Antibo***s are purified by peptide affinity chromatography.
該多克隆抗體是由合成的人源的針對PRAS40 蛋白氨基酸序列的肽段免疫動物而生産的。抗體由肽親和層析技術純化。
The defensin protein was purified by affinity chromatography using the self-cleavable fusion protein.
然後，通過親和層析利用自剪切融合蛋白分離提純目的蛋白。
In the present study, recombinant human IRF-7 was expressed in Escherichia coli, purified by affinity chromatography, and utilized to raise polyclonal antibody in BALB/c mice.
本文在大腸杆菌中表達了重組IRF-7，利用親和層析的方法進行了純化，并制備了鼠抗IRF-7的多克隆抗體。
The trypsin was covalently linked with chemical modified granule chitosan and was used to isolate and purify aprotininum from the extract of cattle lungs by affinity chromatography.
采用瓊脂糖凝膠為載體，共價偶聯牛胰蛋白酶，制成抑肽酶親和吸附劑，将其用于親和層析牛肺提取液，分離純化高比活抑肽酶。
The purity of inclusion bo***s was above 70% and that of protein purified by nickel affinity chromatography was 95%.
提純的包涵體純度可達70%以上，用鎳親和層析方法純化蛋白則可達到95%。
Expressed proteins as inclusion bo***s were solubilized, refolded and purified by GST affinity chromatography.
表達産物為包涵體形式，溶解和折疊後，以GST親合層析色譜純化。
Polyclonal antibo***s are produced by immunizing animals with a synthetic peptide corresponding to human CENP-A protein. Antibo***s are purified by peptide affinity chromatography.
通過合成的與人源CENP-A蛋白相應的多肽片段去免疫動物從而制備出此多克隆抗體。通過多肽親和層析純化獲得。
PurposeThe aim is to purify human platelet factor 4 (PF4) using a monoclonal antibody affinity chromatography.
目的用單克隆抗體親和色譜從人血小闆破碎液中純化血小闆第4因子（PF4 ）。
Affinity chromatography is a powerful tool used in the purification of antibo***s. In this paper, different kinds of affinity ligands used in affinity chromatography are described.
親和色譜是用于抗體純化的有效方法，本文對親和色譜配體的研究進展進行了綜述。
Incorporating with appropriate transport model equation, the SMA model was used to model the dye-ligand affinity chromatography process.
結合適當的傳質模型方程，将SMA模型應用于色素親和色譜過程模拟。
Methods Human sperm mannose receptors were purified by mannose-agarose gel affinity chromatography coloumn.
目的分離純化人精子甘露糖受體并測定其分子量和等電點。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
建立雜交瘤單抗親和層析純化抗原、抗原體外緻敏淋巴細胞和RT-PCR克隆人抗體基因及噬菌體呈現技術構建人源抗體庫的策略。
Along with the development in the very field, novel types of affinity chromatography emerged in recent years, and its range of application expanded significantly as well.
隨着技術的發展，出現了多種新型的親和色譜，其應用範圍也不斷拓展。
Both proteins were purified with Nickel chelate affinity chromatography.
經金屬螯合層析純化，獲得了高純度的表達蛋白。
Highly purified preparation of this protein was obtained through purification with affinity chromatography.
親和層析獲得了高純度的蛋白。
Recombinant human-like collagen with a hexahistidine tail attacked to the carboxyl end could be easily purified by Metal Chelated Affinity Chromatography(MCAC).
研究了應用金屬螯合親和層析法純化重組類人膠原蛋白的條件。
The soluble expression products was purified by 50%(φ) Ni-NTA affinity chromatography and used as an antigen, AIV antibody could be detected by ELISA method.
将可溶表達的融合蛋白用體積分數為50%的N i-NTA樹脂過柱純化，用融合蛋白作抗原，通過ELISA方法，能特異性地檢測出禽流感陽性血清。
Among all purification methods, affinity chromatography shows the excellent characteristics such as high selectivity, high yield, etc.
以親和技術為基礎的分離純化方法與其它分離制備方法相比具有高選擇性、高活力回收等優點。
親和層析（affinity chromatography）是一種基于生物分子間特異性相互作用的分離純化技術。它利用目标分子（如蛋白質、酶或抗體）與固定相中的配體（ligand）之間的選擇性結合能力，例如抗原-抗體、酶-底物、受體-配體等特異性識别機制（來源：Wikipedia）。其核心原理是通過共價結合将配體固定在固相載體（如瓊脂糖微球）表面，當含有目标分子的混合物流經層析柱時，目标分子被配體捕獲，雜質則被洗脫，最後通過改變緩沖液條件（如pH或離子濃度）實現目标分子的高純度分離（來源：Nature Methods）。
該技術的關鍵步驟包括：
在生物醫藥領域，親和層析被廣泛應用于單克隆抗體純化（來源：Thermo Fisher Scientific）、疫苗制備和診斷試劑開發，其分離效率可達普通層析技術的100-1000倍。最新研究顯示，基因工程改造的仿生配體可将層析柱載量提升至50 mg/mL基質（來源：ScienceDirect書籍《Protein Purification》）。
親和色譜法（Affinity Chromatography）是一種基于生物分子間特異性識别能力的分離純化技術，主要用于高效分離目标物質（如蛋白質、酶、抗體等）。以下是詳細解釋：
如需進一步了解操作步驟或具體案例，可參考分析測試百科網及海詞詞典的完整内容。
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