[生化] 黃嘌呤氧化酶
The level of uric acid in plasma and the activities of xanthine oxidase (XO) in the plasma and the small intestine were determined.
試劑盒檢測血漿尿酸含量、血漿及小腸組織黃嘌呤氧化酶（XO）的活性。
Method:Xanthine- xanthine oxidase and trace element selenium were put into the human embryo articular chondrocytes culture liquid in vitro.
方法：在體外培養的人胚關節軟骨細胞培養液中，加入黃嘌呤、黃嘌呤氧化酶及微量元素硒。
Method: The mouse serum uric acid level and liver xanthine oxidase activity were used to evaluate anti-gout effects of raw and processing P. amurense with salt.
方法：以小鼠血清尿酸水平和肝髒黃嘌呤氧化酶活性為指标，評價黃柏生品和鹽制品抗痛風作用。
The content of malondialdehyde (MDA) was measured with thiobarbituric acid method and the activity of superoxide dismutase (SOD) was measured with xanthine oxidase method;
硫代巴比妥酸法測定腦組織丙二醛含量； 黃嘌呤氧化酶法測定************歧化酶活性；
The kit comprises the following main components: buffer solution, coenzyme, inosine, nucleoside phosphatase, xanthine oxidase, NAD(P)H oxidase and a stabilizer.
本發明的試劑盒主要成分包 括：緩沖液、輔酶、肌苷、核苷磷酸酶、黃嘌呤氧化酶、NAD（P）H氧化酶及穩 定劑；
SOD total activity was detected by xanthine oxidase.
SOD試劑盒采用黃嘌呤氧化酶法測其總活力。
Methods: Screening and detecting the active principle which can resist gout of stigmata maydis by using the assay method of xanthine oxidase (XO) in vitro.
方法：利用黃嘌呤氧化酶體外篩選測定方法和平台，檢測并篩選玉米須的抗痛風成分。
Dog xanthine calculus is a peculiar disease, it is caused by the deficiency of innatexanthine oxidase, it has not been reported that is caused by other postnatal factor.
犬黃嘌呤結石症是一種非常罕見的疾病，一般是由于犬體内先天性黃嘌呤氧化酶缺少所緻，因後天因素引起的病例未見報道。
Objective: To study the inhibitory effect of rosmarinic acid on xanthine oxidase .
目的：研究迷疊香酸對黃嘌呤氧化酶的抑制作用。
SOD activities in RBC and liver were measured by xanthine oxidase method. Serum and hepatic MDA contents were assayed according to thiobarbituric acid method.
采用黃嘌呤氧化酶法測定紅細胞和肝勻漿SOD活力，以硫代巴比妥酸法測定血清和肝勻漿MDA含量。
Therefore, the correlation could be expected between serum uric acid levels acting as the markers of both increased xanthine oxidase activity and inflammation.
因此，可預期各血清尿酸值的相關性，而作為黃嘌呤氧化酶活性增強與炎症的指标。
Characters and applications of xanthine oxidase of cow milk were described.
綜述了牛乳中黃嘌呤氧化酶的各種性質及其應用。
Activity of SOD was detected by xanthine oxidase technique .
采用黃嘌呤氧化酶法測定SOD活性；
The results indicated that xanthine oxidase pathway was a main source for the production of oxygen derived free radical.
黃嘌呤氧化酶途徑是體外循環術中氧自由基産生的重要來源。
Methods: Blood concentration of NO, SOD were measured by using nitrate reduction method and xanthine oxidase method.
方法：采用硝酸還原酶法和黃嘌呤氧化酶法測定血漿中************（NO）、************歧化酶（SOD）的水平。
This study aimed to explore the relationship between xanthine oxidase (XO), one of the major sources of ROS, and intestinal injury using a neonatal rat model of NEC.
該研究采用新生鼠壞死性小腸結腸炎模型，探讨黃嘌呤氧化酶與腸損傷的關系。
An immobilization of xanthine oxidase on the surface of microelectrode has been accomplished, and thin film microsensor for the determination of hypoxanthine has been developed in this study.
本項研究實現在微電極表面進行黃嘌呤氧化酶的固定化，從而制備一體化薄膜次黃嘌呤微傳感器。
Objective: To study UV-vis spectral properties of the interaction of quercetin and xanthine oxidase and the effect of incubation time on the inhibition of xanthine oxidase.
目的：研究了槲皮素與黃嘌呤氧化酶相互作用的紫外光譜性質及孵育時間對酶抑制性的影響。
Results: In xanthine oxidase method, the specific activity of SOD still could be detected although SOD was inactivated.
結果：在黃嘌呤氧化酶法反應體系中，SOD滅活後依然能夠檢測到SOD比活力值。
The reagent (kit) mainly comprises the following components: a buffer solution, coenzyme, uric acid, amino acid oxidase, xanthine oxidase, xanthine dehydrogenase and a stabilizer.
本發明的試劑（盒）主要成分包括：緩沖液、輔酶、尿酸、氨基酸氧化酶、黃嘌呤氧化酶、黃嘌呤脫氫酶及穩定劑；
The serum was collected and added into the reaction system of xanthine, xanthine oxidase and ESR spin trap 5,5 dimethylpyroline N oxide to assay the levels of superoxide dismutase (SOD) by ESR.
取血清，加入黃嘌呤，黃嘌呤氧化酶及自旋共振自旋捕集劑*********吡啶氮氧化物以判斷血清中************歧化酶（SOD）含量變化。
It can regulate and repair the nerve cells of human body thoroughly, and prevent the syndromes of climacterium. it can effectively inhibit xanthine oxidase, and decrease the formation of uric acid;
能全面調節和修複人體的神經細胞，防治更年期綜合征。可有效地抑制黃嘌呤氧化酶，使尿酸生成減少；
Methods: Blood samples of 30 GDM and 45 unrelated normal controls were used to measure MDA, SOD and NO levels with methods of TBA, xanthine oxidase and nitric reductase.
方法：采集30例GDM患者和4 5例正常孕婦血清，以硫代巴比妥酸（TBA）比色法、黃嘌呤氧化酶法、硝酸還原酶法分别測定MDA ，SOD和NO含量。
黃嘌呤氧化酶（xanthine oxidase）是一種含钼的黃素蛋白酶，廣泛存在于生物體的肝髒、腸道和乳腺等組織中。該酶在嘌呤代謝途徑中發揮關鍵作用，主要催化黃嘌呤向尿酸的氧化反應，同時将分子氧還原為過氧化氫或超氧化物（來源：《生物化學雜志》第298卷）。其活性中心的钼原子通過氧化态變化實現電子轉移，這一機制在2014年《自然》期刊的蛋白質結構解析研究中得到詳細闡述。
從生理功能角度看，黃嘌呤氧化酶不僅參與核酸分解代謝，還涉及活性氧（ROS）的生成。美國國立衛生研究院（NIH）的臨床研究表明，該酶的異常活化與痛風、慢性心力衰竭等疾病密切相關。例如，當尿酸生成過量時，可能引發高尿酸血症和痛風性關節炎（來源：《新英格蘭醫學雜志》2017年臨床綜述）。
在藥物研發領域，黃嘌呤氧化酶抑制劑（如别嘌呤醇）已成為痛風治療的一線用藥。2022年《柳葉刀》發表的隨機對照試驗證實，新型選擇性抑制劑Febuxostat較傳統藥物具有更優的療效和安全性。值得關注的是，乳制品中的黃嘌呤氧化酶可能通過腸道吸收影響人體代謝，這一現象在《營養學進展》的多中心隊列研究中被持續追蹤。
黃嘌呤氧化酶（Xanthine Oxidase）是一種重要的含钼黃素蛋白酶，其功能與嘌呤代謝密切相關。以下是綜合多來源的詳細解釋：
1. 定義與組成
黃嘌呤氧化酶屬于氧化還原酶類，以黃嘌呤和次黃嘌呤為底物，催化其氧化反應。它含有钼輔因子和鐵硫中心，是典型的金屬黃素蛋白。
2. 催化反應
其核心功能包括：
3. 生理與病理作用
4. 應用與抑制劑
術語擴展
英文别稱包括XOD（Xanthine Oxidoreductase），其功能多樣性還涉及醛類氧化。
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