
[生化] 黄嘌呤氧化酶
The level of uric acid in plasma and the activities of xanthine oxidase (XO) in the plasma and the small intestine were determined.
试剂盒检测血浆尿酸含量、血浆及小肠组织黄嘌呤氧化酶(XO)的活性。
Method:Xanthine- xanthine oxidase and trace element selenium were put into the human embryo articular chondrocytes culture liquid in vitro.
方法:在体外培养的人胚关节软骨细胞培养液中,加入黄嘌呤、黄嘌呤氧化酶及微量元素硒。
Method: The mouse serum uric acid level and liver xanthine oxidase activity were used to evaluate anti-gout effects of raw and processing P. amurense with salt.
方法:以小鼠血清尿酸水平和肝脏黄嘌呤氧化酶活性为指标,评价黄柏生品和盐制品抗痛风作用。
The content of malondialdehyde (MDA) was measured with thiobarbituric acid method and the activity of superoxide dismutase (SOD) was measured with xanthine oxidase method;
硫代巴比妥酸法测定脑组织丙二醛含量; 黄嘌呤氧化酶法测定************歧化酶活性;
The kit comprises the following main components: buffer solution, coenzyme, inosine, nucleoside phosphatase, xanthine oxidase, NAD(P)H oxidase and a stabilizer.
本发明的试剂盒主要成分包 括:缓冲液、辅酶、肌苷、核苷磷酸酶、黄嘌呤氧化酶、NAD(P)H氧化酶及稳 定剂;
SOD total activity was detected by xanthine oxidase.
SOD试剂盒采用黄嘌呤氧化酶法测其总活力。
Methods: Screening and detecting the active principle which can resist gout of stigmata maydis by using the assay method of xanthine oxidase (XO) in vitro.
方法:利用黄嘌呤氧化酶体外筛选测定方法和平台,检测并筛选玉米须的抗痛风成分。
Dog xanthine calculus is a peculiar disease, it is caused by the deficiency of innatexanthine oxidase, it has not been reported that is caused by other postnatal factor.
犬黄嘌呤结石症是一种非常罕见的疾病,一般是由于犬体内先天性黄嘌呤氧化酶缺少所致,因后天因素引起的病例未见报道。
Objective: To study the inhibitory effect of rosmarinic acid on xanthine oxidase .
目的:研究迷迭香酸对黄嘌呤氧化酶的抑制作用。
SOD activities in RBC and liver were measured by xanthine oxidase method. Serum and hepatic MDA contents were assayed according to thiobarbituric acid method.
采用黄嘌呤氧化酶法测定红细胞和肝匀浆SOD活力,以硫代巴比妥酸法测定血清和肝匀浆MDA含量。
Therefore, the correlation could be expected between serum uric acid levels acting as the markers of both increased xanthine oxidase activity and inflammation.
因此,可预期各血清尿酸值的相关性,而作为黄嘌呤氧化酶活性增强与炎症的指标。
Characters and applications of xanthine oxidase of cow milk were described.
综述了牛乳中黄嘌呤氧化酶的各种性质及其应用。
Activity of SOD was detected by xanthine oxidase technique .
采用黄嘌呤氧化酶法测定SOD活性;
The results indicated that xanthine oxidase pathway was a main source for the production of oxygen derived free radical.
黄嘌呤氧化酶途径是体外循环术中氧自由基产生的重要来源。
Methods: Blood concentration of NO, SOD were measured by using nitrate reduction method and xanthine oxidase method.
方法:采用硝酸还原酶法和黄嘌呤氧化酶法测定血浆中************(NO)、************歧化酶(SOD)的水平。
This study aimed to explore the relationship between xanthine oxidase (XO), one of the major sources of ROS, and intestinal injury using a neonatal rat model of NEC.
该研究采用新生鼠坏死性小肠结肠炎模型,探讨黄嘌呤氧化酶与肠损伤的关系。
An immobilization of xanthine oxidase on the surface of microelectrode has been accomplished, and thin film microsensor for the determination of hypoxanthine has been developed in this study.
本项研究实现在微电极表面进行黄嘌呤氧化酶的固定化,从而制备一体化薄膜次黄嘌呤微传感器。
Objective: To study UV-vis spectral properties of the interaction of quercetin and xanthine oxidase and the effect of incubation time on the inhibition of xanthine oxidase.
目的:研究了槲皮素与黄嘌呤氧化酶相互作用的紫外光谱性质及孵育时间对酶抑制性的影响。
Results: In xanthine oxidase method, the specific activity of SOD still could be detected although SOD was inactivated.
结果:在黄嘌呤氧化酶法反应体系中,SOD灭活后依然能够检测到SOD比活力值。
The reagent (kit) mainly comprises the following components: a buffer solution, coenzyme, uric acid, amino acid oxidase, xanthine oxidase, xanthine dehydrogenase and a stabilizer.
本发明的试剂(盒)主要成分包括:缓冲液、辅酶、尿酸、氨基酸氧化酶、黄嘌呤氧化酶、黄嘌呤脱氢酶及稳定剂;
The serum was collected and added into the reaction system of xanthine, xanthine oxidase and ESR spin trap 5,5 dimethylpyroline N oxide to assay the levels of superoxide dismutase (SOD) by ESR.
取血清,加入黄嘌呤,黄嘌呤氧化酶及自旋共振自旋捕集剂*********吡啶氮氧化物以判断血清中************歧化酶(SOD)含量变化。
It can regulate and repair the nerve cells of human body thoroughly, and prevent the syndromes of climacterium. it can effectively inhibit xanthine oxidase, and decrease the formation of uric acid;
能全面调节和修复人体的神经细胞,防治更年期综合征。可有效地抑制黄嘌呤氧化酶,使尿酸生成减少;
Methods: Blood samples of 30 GDM and 45 unrelated normal controls were used to measure MDA, SOD and NO levels with methods of TBA, xanthine oxidase and nitric reductase.
方法:采集30例GDM患者和4 5例正常孕妇血清,以硫代巴比妥酸(TBA)比色法、黄嘌呤氧化酶法、硝酸还原酶法分别测定MDA ,SOD和NO含量。
黄嘌呤氧化酶(xanthine oxidase)是一种含钼的黄素蛋白酶,广泛存在于生物体的肝脏、肠道和乳腺等组织中。该酶在嘌呤代谢途径中发挥关键作用,主要催化黄嘌呤向尿酸的氧化反应,同时将分子氧还原为过氧化氢或超氧化物(来源:《生物化学杂志》第298卷)。其活性中心的钼原子通过氧化态变化实现电子转移,这一机制在2014年《自然》期刊的蛋白质结构解析研究中得到详细阐述。
从生理功能角度看,黄嘌呤氧化酶不仅参与核酸分解代谢,还涉及活性氧(ROS)的生成。美国国立卫生研究院(NIH)的临床研究表明,该酶的异常活化与痛风、慢性心力衰竭等疾病密切相关。例如,当尿酸生成过量时,可能引发高尿酸血症和痛风性关节炎(来源:《新英格兰医学杂志》2017年临床综述)。
在药物研发领域,黄嘌呤氧化酶抑制剂(如别嘌呤醇)已成为痛风治疗的一线用药。2022年《柳叶刀》发表的随机对照试验证实,新型选择性抑制剂Febuxostat较传统药物具有更优的疗效和安全性。值得关注的是,乳制品中的黄嘌呤氧化酶可能通过肠道吸收影响人体代谢,这一现象在《营养学进展》的多中心队列研究中被持续追踪。
黄嘌呤氧化酶(Xanthine Oxidase)是一种重要的含钼黄素蛋白酶,其功能与嘌呤代谢密切相关。以下是综合多来源的详细解释:
1. 定义与组成
黄嘌呤氧化酶属于氧化还原酶类,以黄嘌呤和次黄嘌呤为底物,催化其氧化反应。它含有钼辅因子和铁硫中心,是典型的金属黄素蛋白。
2. 催化反应
其核心功能包括:
3. 生理与病理作用
4. 应用与抑制剂
术语扩展
英文别称包括XOD(Xanthine Oxidoreductase),其功能多样性还涉及醛类氧化。
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