[生化] 聚合酶鍊反應
Objective: To investigate the clinical value of Polymerase Chain Reaction in the diagnosis of trachoma.
目的：探讨聚合酶鍊反應在沙眼診斷中的臨床應用價值。
To investigate the application of the polymerase chain reaction (PCR) in diagnosis of oral candida leukoplakia.
探讨聚合酶鍊反應技術在檢測口腔白斑中念珠菌感染的應用。
METHODS:The 5HT2A receptor gene T102C polymorphism was analyzed for genotyping by the method of polymerase chain reaction(PCR) in 177 male patients with schiz phrenia of different areas.
方法：采用聚合酶鍊反應的方法對不同省區的177例男性精神分裂症患者5HT2A受體基因T102C多态進行基因型分析。
Polymerase Chain Reaction(PCR)as an efficient DNA amplification technique in vitro hasbeen widely al (?) lied to clinical gene diagnosis of some hereditary diseases.
聚合酶鍊反應作為一種強有效的DNA 體外擴增技術，已被廣泛地應用于一些遺傳性疾病的臨床基因診斷。
Meanwhile, biochemical reaction, coagglutination test, metabolism-inhibition test, polymerase chain reaction (PCR), and DNA sequence assay were employed to identify those positive cultures.
陽性培養物用生化反應、協同凝集試驗、代謝抑制試驗、聚合酶鍊反應和DNA測序來加以鑒定。
We discussed on the principle and specialty of PCR(Polymerase Chain Reaction) and stu***d on its applications in detection of food microorganism and genetically modified food.
簡述了聚合酶鍊反應（PCR）技術的基本原理、特點及其在食品微生物檢測、轉基因食品檢驗等食品工程領域的應用情況。
Methods:Karyotype analysis and SRY gene in twenty patients were analyzed by the technique of chromosomal G band and polymerase chain reaction(PCR).
方法應用染色體G顯帶與聚合酶鍊反應對性分化異常患者進行染色體核型分析與SRY基因的檢測。
Methods Exon 4~9 of PTEN were amplified by polymerase chain reaction (PCR) for homozygous deletions in 110 tumor samples of patients with bone and soft tissue tumors, 3 tumor cell lines.
方法應用聚合酶鍊反應（PCR）分析骨及軟組織腫瘤患者110例腫瘤标本，3個腫瘤細胞系PTEN基因第4～9外顯子有無純合性缺失。
Methods The identification of VNTR polymorphism of the glycoprotein Ibαgene was performed by polymerase chain reaction (PCR).
方法聚合酶鍊反應擴增目的片斷，根據産物的長度判斷VNTR基因型。
METHODS: Method of multiplex polymerase chain reaction(PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
方法：采用多重聚合酶鍊反應方法，以提取的基因組DNA為模闆，在同一反應管中同時擴增細胞周期素D1基因和P16基因。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
目的：利用聚合酶鍊反應定點突變技術構建人血小闆反應素1基因第13外顯子編碼鈣結合域突變體。
Methods Polymerase chain reaction(PCR).
方法聚合酶鍊反應。
A panel of 24 oral fluids was used to investigate if measles virus haemagglutinin (H) and nucleocapsid (N) genes could be amplified by polymerase chain reaction directly from used POCT strips.
通過聚合酶鍊反應對一組24個使用過的床旁檢測測試條上的唾液标本進行檢測，分析麻疹病毒血凝素（）和核蛋白（N）基因是否可以直接檢出。
Polymerase chain reaction (PCR).
聚合酶鍊反應。
Methods Cytogenetic analysis and multiplex polymerase chain reaction(PCR) analysis were done on the 148 patients with azoospermia and serious oligozoospermia.
方法應用細胞遺傳學和多重聚合酶鍊反應技術，對148例無精症、嚴重少精症患者進行檢測。
Methods Using reverse transcription-polymerase chain reaction(RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法應用逆轉錄-聚合酶鍊反應及免疫組化檢測PIM3在小鼠眼部組織中的表達。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR)in detection of HCV RNA in serum and peripheral blood monocular cells(PBMC).
目的探讨實時熒光定量聚合酶鍊反應（FQ-PCR）在檢測外周血清及單核細胞中HCVRNA含量的臨床應用價值。
Polymerase Chain Reaction(PCR)technique has been widely used in detecting different hepatitis viruses in laboratory, because of its high specificity and sensitivity.
聚合酶鍊反應（PCR）技術用于病毒核酸的檢測具有靈敏度高，特異性強等優點，已廣泛用于各型肝炎病毒的實驗室檢測。
Mehtods A case control study was carried out and polymerase chain reaction (PCR)technique was used to identify GSTM1, GSTT1 genotype in 89 cases of primary gastric cancer and 94 controls.
方法采用病例對照分子流行病學研究方法和聚合酶鍊反應技術，檢測89例原發性胃癌病例和94例對照GSTM 1和GSTT1基因型。
Current multiplex polymerase chain reaction (PCR) techniques can at most offer detection from among 50 organisms in one test.
現行的多元聚合酶鍊反應（PCR）技術最多隻能在50種生物内進行一次檢測。
PCR The short name for polymerase chain reaction, a sensitive lab test that can measure the presence of cancer cell-markers in the blood or marrow.
“聚合酶鍊式反應”的簡稱，一種可在血液或骨髓中檢測到癌細胞标志物存在的靈敏的實驗室試驗。
Objective:To evaluate the diagnostic value of polymerase chain reaction (PCR), culture, Semar acid fast staining, fluorescent staining in tuberculosis.
目的：評價聚合酶鍊反應（PCR） ，細菌培養，抗酸染色，熒光染色對結核病的診斷價值。
Diagnostics of infectious deseases and deseases of the immune system in serology and molecular biology - Part 60: Polymerase chain reaction (PCR); Terminology, general method-specific requirements.
傳染病和免疫性疾病的血清學診斷和分子生物學診斷。第60部分：聚合酶鍊式反應。術語、一般方法和特殊要求。
Objective To investigate the fluorescence quantitative polymerase chain reaction(FQ-PCR)in the giant cell viral hepatitis in value.
目的探讨熒光定量聚合酶鍊反應（FQ-PCR）在巨細胞病毒性肝炎中的應用價值。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
采用單鍊聚合酶鍊反應（PCR）和測序法檢測腫瘤組織中VHL基因的突變情況。
This technique employs standard operations of biomedical engineering, such as polymerase chain reaction(PCR), agarose gel electrophoresis, labeling and detecting of probe.
需要使用包括聚合酶鍊反應（PCR），瓊脂糖凝膠電泳，探針的标記與檢測等生物工程技術。
Objective:Evaluation of methylation specific polymerase chain reaction and its potential clinical value in the detection of minimal residual disease (MRD) in ***** acute leukemia with P15.
目的：評價由甲基化特異性聚合酶鍊反應分析P15 基因甲基化對成人急性白血病微小殘留病竈的檢測價值。
Objective To investigate the value of duplex polymerase chain reaction (DPCR) in early diagnosis of Legionella pneumonia by detecting Legionella DNA in sputum and bronchoalvelar lavage fluid(BALF).
目的探讨雙重聚合酶鍊反應（DPCR）法檢測痰及支氣管肺泡灌洗液（BALF）中軍團菌DNA在早期診斷軍團菌肺炎的意義。
聚合酶鍊式反應（Polymerase Chain Reaction，PCR）是一種在體外快速擴增特定DNA片段的技術，由Kary Mullis于1983年發明，被譽為分子生物學領域的革命性突破。其核心原理是通過溫度調控，模拟DNA自然複制過程，實現目标序列的指數級擴增。
以上步驟循環25–40次，理論上可使目标DNA量呈指數增長（每循環翻倍）。
自經典PCR問世後，衍生出多種改進技術：
該技術因高效、靈敏、特異性強，已成為現代生命科學實驗室的标配工具，其發明者Mullis也因此獲得1993年諾貝爾化學獎。
聚合酶鍊反應是一種用于擴增DNA序列的技術。下面是對該詞的詳細解釋：
聚合酶鍊反應是一種先進的生物技術，它能夠在短時間内擴增少量的DNA序列，從而使其得到足夠的分析。PCR常被用于DNA檢測、診斷和遺傳學研究等領域。
聚合酶鍊反應是通過在不斷變化的溫度條件下，複制和擴增DNA分子。該技術利用聚合酶酶（polymerase）将DNA鍊延長，從而擴增DNA序列。PCR技術通常包括三個步驟：變性、退火和延伸。在變性步驟中，DNA雙螺旋結構被分離成兩個單鍊。在退火步驟中，引物（primers）與DNA模闆結合，并啟動聚合酶鍊反應。在延伸步驟中，聚合酶酶将引物延伸到DNA鍊上，從而使其得到擴增。
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