
酒精沉澱法
The pumpkin pectin was obtained by alcohol precipitation after ultrafiltration.
本試驗采用超濾濃縮後乙醇沉澱制備南瓜果膠。
Crude polysaccharide was gained by hot water extraction, alcohol precipitation, decolorization and deproteinization.
以莪術為材料,經熱水提取、酒精沉澱、脫色、脫蛋白得粗多糖。
Acid mucopolysaccharides were prepared from the leftover bits of scallop with protein enzyme hydrolyzation and alcohol precipitation.
通過蛋白酶水解和乙醇沉澱,從扇貝柱加工下腳料(扇貝邊)中提取酸性粘多糖。
METHODS Combined water extract and alcohol precipitation, herbs was administrated after sterilization.
方法:采用中藥水提酒精沉澱消毒後腹腔灌洗。
Results Utilizing lactic acid dissolution and alcohol precipitation technology could prepare granules of chitosan lactate, which had characteristic absorption peak in infrared spectrum.
結果利用乳酸溶解和醇沉澱工藝可制備出殼聚糖乳酸鹽顆粒,在紅外光譜中具有特征性吸收峰。
Experimental Methodsare: Crude polysaccharide was gained through hot water extraction, alcohol precipitation, decolorization and deproteinization.
莪術經熱水提取,酒精沉澱,脫色,脫蛋白得粗多糖。
MethodsThe PTPS was obtained by water extraction, alcohol precipitation, and DEAE-cellulose column.
方法應用水提、乙醇沉澱、DEAE-纖維素色譜,對PTPS進行提取并分級;
Humic acid extracted from peat was classified into seven fractions by alcohol precipitation technique for the analysis of thermolysis, flocculation limit and some optical properties.
結果表明,酒精沉澱分級法是按分子量大小、縮合程度的高低将胡敏酸分成較均一的級分,而對胡敏酸的性質、結構等無破壞作用。
In this paper, high methoxyl pectin was extracted from apple residue by method of acid solution and alcohol precipitation.
以蘋果渣為原料,采取酸液提取、酒精沉析的方法制取高*********果膠。
Methods:Flocculating method and alcohol precipitation meth od were used.
方法:采用了醇沉法和絮凝法兩種精制方法。
METHODS Using the content of ferulic acid as index, the optimum extraction conditions of water extraction and alcohol precipitation was stu***d with the orthogonal test of L9(34).
方法:以阿魏酸含量為考察指标,通過L9(34)正交試驗設計優選水提醇沉的制備工藝條件,确定醒腦益智顆粒的最佳制備工藝。
Methods:Oral liquid was made by steam distillation, alcohol abstraction method, water abstraction and alcohol precipitation.
方法:分别采用水蒸氣蒸餾法、醇提法、水提醇沉法制成口服液;
The crude polysaccharide was extracted from Laminari japonica by ethyl alcohol precipitation method, and then it was treated with AB-80 macroporous absorptive resin.
乙醇沉澱法提取海帶硫酸多糖,用AB-80大孔吸附樹脂柱去除色素。
To improve the functionalities of soy protein concentrate made by alcohol precipitation, physical modification with microwave was stu***d.
為改善醇法大豆濃縮蛋白的功能特性,采用微波技術對醇法大豆濃縮蛋白進行物理改性。
The adaptation and technological parameters of ceramic membrane were investigated and compared with the traditional technique of water extraction and alcohol precipitation.
方法以蝙蝠蛾被毛孢菌絲體水提液為對象,考察陶瓷膜的適配性與工藝參數,并與傳統的水提醇沉技術對比。
The water-soluble Enteromorpha polysaccharides (EP) were obtained through aqueous extraction-alcohol precipitation technology.
用熱水直接提取、乙醇沉澱的方法提取出浒苔水溶性多糖;
Objective: The effect of separation rates on the quality and shelf life of TCM in the process of alcohol precipitation was discussed.
目的:簡要讨論醇沉過程中分離程度對中藥質量、保質期等的影響。
Objective:To compare the fit and unfit of alcohol precipitation process and chitosan flocculation process used in purification of extract of yanyan buccal tablets.
目的:比較醇沉法和殼聚糖絮凝法用于咽炎口含片浸膏純化的優劣。
OBJECTIVE To determine the alcohol precipitation techniques for polystictus versicolor polysaccharides.
目的确定雲芝多糖的醇沉工藝。
Water extraction with alcohol precipitation was used to prepare for Angelica.
殘渣經水煮、醇沉提取當歸多糖;
The separation of 1,3-propanediol from fermentative broth by a combination of ultrafiltration and alcohol precipitation was well investigated.
介紹了超濾-醇沉法從發酵液中提取1,3-*********的工藝。
The process conditions of separation and purification via diatom earth filtration and alcohol precipitation were stu***d .
醇析法是一種有效分離提純黃原膠的方法。
Conclusion:The process of flocculation precipitation is better than the process of alcohol precipitation according to the removal rate of harmful elements and reserved rate of useful elements.
結論:從有害元素的去除率及有益元素的保留率來看,絮沉工藝優于醇沉工藝。
The loss rate of sacidrosiele by using flocculating method is less than that by using alcohol precipitation.
絮凝法紅景天總皂苷損失率明顯低于醇沉法,且殼聚糖吸附法的效果最佳。
Objective: To study the effects of water extraction, water extract-alcohol precipitation and ethanol ultrasonic extraction on the content of astragalus saponin.
目的:研究水提法、水提醇沉法、乙醇超生提取法三種提取方法對黃芪總皂苷的影響。
Methods Heparin of injection level was treated through degradation by sodium nitrite, desalting by 732 ion exchange resin, neutralization with calcium oxide, separation with alcohol precipitation.
方法以市售注射用肝素鈉為原料 ,經過************降解成低分子肝素鈉 ,樹脂脫鈉 ,氧化鈣中和轉型成鈣鹽 ,最後用乙醇沉澱析出。
Tannin fractions with different molecular weight distributions were obtained by purification of alcohol precipitation and fractionation of solvent extractions of black wattle bark tannins.
通過對黑荊樹皮單甯進行醇沉純化和溶劑萃取分級 ,可得到分子量分布範圍不同的單甯級分。
酒精沉澱法(Alcohol Precipitation)是一種基于溶液極性變化的分離技術,廣泛應用于生物化學和分子生物學領域。該方法通過向溶液中加入乙醇、異丙醇等有機溶劑,降低目标物質(如核酸、多糖或蛋白質)的溶解度,促使其形成固态沉澱物,從而實現純化或濃縮。
酒精通過改變溶液的介電常數和氫鍵作用力,破壞溶質分子周圍的水合層。例如在DNA純化中,高濃度乙醇(通常70%-80%)會削弱DNA分子與水分子間的相互作用,促使DNA鍊間通過磷酸骨架的負電荷與陽離子(如鈉離子)結合,聚集成可見沉澱。這一過程符合《分子克隆實驗指南》中闡述的電荷屏蔽原理。
實驗效果受三因素顯著影響:
該技術作為基礎分離手段,其标準化操作流程已被國際純粹與應用化學聯合會(IUPAC)收錄為推薦實驗方法。
“Alcohol precipitation”是一個化學領域術語,結合了“alcohol(醇類)”和“precipitation(沉澱)”的雙重含義,指通過加入醇類物質(如乙醇)促使溶液中的特定成分形成沉澱的技術。以下是詳細解釋:
典型的醇沉法(alcohol precipitation procedure)包括以下步驟:
如需具體案例或擴展應用場景,可參考相關化學實驗手冊或生物技術文獻。
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