
酒精沉淀法
The pumpkin pectin was obtained by alcohol precipitation after ultrafiltration.
本试验采用超滤浓缩后乙醇沉淀制备南瓜果胶。
Crude polysaccharide was gained by hot water extraction, alcohol precipitation, decolorization and deproteinization.
以莪术为材料,经热水提取、酒精沉淀、脱色、脱蛋白得粗多糖。
Acid mucopolysaccharides were prepared from the leftover bits of scallop with protein enzyme hydrolyzation and alcohol precipitation.
通过蛋白酶水解和乙醇沉淀,从扇贝柱加工下脚料(扇贝边)中提取酸性粘多糖。
METHODS Combined water extract and alcohol precipitation, herbs was administrated after sterilization.
方法:采用中药水提酒精沉淀消毒后腹腔灌洗。
Results Utilizing lactic acid dissolution and alcohol precipitation technology could prepare granules of chitosan lactate, which had characteristic absorption peak in infrared spectrum.
结果利用乳酸溶解和醇沉淀工艺可制备出壳聚糖乳酸盐颗粒,在红外光谱中具有特征性吸收峰。
Experimental Methodsare: Crude polysaccharide was gained through hot water extraction, alcohol precipitation, decolorization and deproteinization.
莪术经热水提取,酒精沉淀,脱色,脱蛋白得粗多糖。
MethodsThe PTPS was obtained by water extraction, alcohol precipitation, and DEAE-cellulose column.
方法应用水提、乙醇沉淀、DEAE-纤维素色谱,对PTPS进行提取并分级;
Humic acid extracted from peat was classified into seven fractions by alcohol precipitation technique for the analysis of thermolysis, flocculation limit and some optical properties.
结果表明,酒精沉淀分级法是按分子量大小、缩合程度的高低将胡敏酸分成较均一的级分,而对胡敏酸的性质、结构等无破坏作用。
In this paper, high methoxyl pectin was extracted from apple residue by method of acid solution and alcohol precipitation.
以苹果渣为原料,采取酸液提取、酒精沉析的方法制取高*********果胶。
Methods:Flocculating method and alcohol precipitation meth od were used.
方法:采用了醇沉法和絮凝法两种精制方法。
METHODS Using the content of ferulic acid as index, the optimum extraction conditions of water extraction and alcohol precipitation was stu***d with the orthogonal test of L9(34).
方法:以阿魏酸含量为考察指标,通过L9(34)正交试验设计优选水提醇沉的制备工艺条件,确定醒脑益智颗粒的最佳制备工艺。
Methods:Oral liquid was made by steam distillation, alcohol abstraction method, water abstraction and alcohol precipitation.
方法:分别采用水蒸气蒸馏法、醇提法、水提醇沉法制成口服液;
The crude polysaccharide was extracted from Laminari japonica by ethyl alcohol precipitation method, and then it was treated with AB-80 macroporous absorptive resin.
乙醇沉淀法提取海带硫酸多糖,用AB-80大孔吸附树脂柱去除色素。
To improve the functionalities of soy protein concentrate made by alcohol precipitation, physical modification with microwave was stu***d.
为改善醇法大豆浓缩蛋白的功能特性,采用微波技术对醇法大豆浓缩蛋白进行物理改性。
The adaptation and technological parameters of ceramic membrane were investigated and compared with the traditional technique of water extraction and alcohol precipitation.
方法以蝙蝠蛾被毛孢菌丝体水提液为对象,考察陶瓷膜的适配性与工艺参数,并与传统的水提醇沉技术对比。
The water-soluble Enteromorpha polysaccharides (EP) were obtained through aqueous extraction-alcohol precipitation technology.
用热水直接提取、乙醇沉淀的方法提取出浒苔水溶性多糖;
Objective: The effect of separation rates on the quality and shelf life of TCM in the process of alcohol precipitation was discussed.
目的:简要讨论醇沉过程中分离程度对中药质量、保质期等的影响。
Objective:To compare the fit and unfit of alcohol precipitation process and chitosan flocculation process used in purification of extract of yanyan buccal tablets.
目的:比较醇沉法和壳聚糖絮凝法用于咽炎口含片浸膏纯化的优劣。
OBJECTIVE To determine the alcohol precipitation techniques for polystictus versicolor polysaccharides.
目的确定云芝多糖的醇沉工艺。
Water extraction with alcohol precipitation was used to prepare for Angelica.
残渣经水煮、醇沉提取当归多糖;
The separation of 1,3-propanediol from fermentative broth by a combination of ultrafiltration and alcohol precipitation was well investigated.
介绍了超滤-醇沉法从发酵液中提取1,3-*********的工艺。
The process conditions of separation and purification via diatom earth filtration and alcohol precipitation were stu***d .
醇析法是一种有效分离提纯黄原胶的方法。
Conclusion:The process of flocculation precipitation is better than the process of alcohol precipitation according to the removal rate of harmful elements and reserved rate of useful elements.
结论:从有害元素的去除率及有益元素的保留率来看,絮沉工艺优于醇沉工艺。
The loss rate of sacidrosiele by using flocculating method is less than that by using alcohol precipitation.
絮凝法红景天总皂苷损失率明显低于醇沉法,且壳聚糖吸附法的效果最佳。
Objective: To study the effects of water extraction, water extract-alcohol precipitation and ethanol ultrasonic extraction on the content of astragalus saponin.
目的:研究水提法、水提醇沉法、乙醇超生提取法三种提取方法对黄芪总皂苷的影响。
Methods Heparin of injection level was treated through degradation by sodium nitrite, desalting by 732 ion exchange resin, neutralization with calcium oxide, separation with alcohol precipitation.
方法以市售注射用肝素钠为原料 ,经过************降解成低分子肝素钠 ,树脂脱钠 ,氧化钙中和转型成钙盐 ,最后用乙醇沉淀析出。
Tannin fractions with different molecular weight distributions were obtained by purification of alcohol precipitation and fractionation of solvent extractions of black wattle bark tannins.
通过对黑荆树皮单宁进行醇沉纯化和溶剂萃取分级 ,可得到分子量分布范围不同的单宁级分。
酒精沉淀法(Alcohol Precipitation)是一种基于溶液极性变化的分离技术,广泛应用于生物化学和分子生物学领域。该方法通过向溶液中加入乙醇、异丙醇等有机溶剂,降低目标物质(如核酸、多糖或蛋白质)的溶解度,促使其形成固态沉淀物,从而实现纯化或浓缩。
酒精通过改变溶液的介电常数和氢键作用力,破坏溶质分子周围的水合层。例如在DNA纯化中,高浓度乙醇(通常70%-80%)会削弱DNA分子与水分子间的相互作用,促使DNA链间通过磷酸骨架的负电荷与阳离子(如钠离子)结合,聚集成可见沉淀。这一过程符合《分子克隆实验指南》中阐述的电荷屏蔽原理。
实验效果受三因素显著影响:
该技术作为基础分离手段,其标准化操作流程已被国际纯粹与应用化学联合会(IUPAC)收录为推荐实验方法。
“Alcohol precipitation”是一个化学领域术语,结合了“alcohol(醇类)”和“precipitation(沉淀)”的双重含义,指通过加入醇类物质(如乙醇)促使溶液中的特定成分形成沉淀的技术。以下是详细解释:
典型的醇沉法(alcohol precipitation procedure)包括以下步骤:
如需具体案例或扩展应用场景,可参考相关化学实验手册或生物技术文献。
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