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alcohol precipitation是什么意思,alcohol precipitation的意思翻译、用法、同义词、例句

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常用词典

  • 酒精沉淀法

  • 例句

  • The pumpkin pectin was obtained by alcohol precipitation after ultrafiltration.

    本试验采用超滤浓缩后乙醇沉淀制备南瓜果胶。

  • Crude polysaccharide was gained by hot water extraction, alcohol precipitation, decolorization and deproteinization.

    以莪术为材料,经热水提取、酒精沉淀、脱色、脱蛋白得粗多糖。

  • Acid mucopolysaccharides were prepared from the leftover bits of scallop with protein enzyme hydrolyzation and alcohol precipitation.

    通过蛋白酶水解和乙醇沉淀,从扇贝柱加工下脚料(扇贝边)中提取酸性粘多糖。

  • METHODS Combined water extract and alcohol precipitation, herbs was administrated after sterilization.

    方法:采用中药水提酒精沉淀消毒后腹腔灌洗。

  • Results Utilizing lactic acid dissolution and alcohol precipitation technology could prepare granules of chitosan lactate, which had characteristic absorption peak in infrared spectrum.

    结果利用乳酸溶解和醇沉淀工艺可制备出壳聚糖乳酸盐颗粒,在红外光谱中具有特征性吸收峰。

  • Experimental Methodsare: Crude polysaccharide was gained through hot water extraction, alcohol precipitation, decolorization and deproteinization.

    莪术经热水提取,酒精沉淀,脱色,脱蛋白得粗多糖。

  • MethodsThe PTPS was obtained by water extraction, alcohol precipitation, and DEAE-cellulose column.

    方法应用水提、乙醇沉淀、DEAE-纤维素色谱,对PTPS进行提取并分级;

  • Humic acid extracted from peat was classified into seven fractions by alcohol precipitation technique for the analysis of thermolysis, flocculation limit and some optical properties.

    结果表明,酒精沉淀分级法是按分子量大小、缩合程度的高低将胡敏酸分成较均一的级分,而对胡敏酸的性质、结构等无破坏作用。

  • In this paper, high methoxyl pectin was extracted from apple residue by method of acid solution and alcohol precipitation.

    以苹果渣为原料,采取酸液提取、酒精沉析的方法制取高*********果胶。

  • Methods:Flocculating method and alcohol precipitation meth od were used.

    方法:采用了醇沉法和絮凝法两种精制方法。

  • METHODS Using the content of ferulic acid as index, the optimum extraction conditions of water extraction and alcohol precipitation was stu***d with the orthogonal test of L9(34).

    方法:以阿魏酸含量为考察指标,通过L9(34)正交试验设计优选水提醇沉的制备工艺条件,确定醒脑益智颗粒的最佳制备工艺。

  • Methods:Oral liquid was made by steam distillation, alcohol abstraction method, water abstraction and alcohol precipitation.

    方法:分别采用水蒸气蒸馏法、醇提法、水提醇沉法制成口服液;

  • The crude polysaccharide was extracted from Laminari japonica by ethyl alcohol precipitation method, and then it was treated with AB-80 macroporous absorptive resin.

    乙醇沉淀法提取海带硫酸多糖,用AB-80大孔吸附树脂柱去除色素。

  • To improve the functionalities of soy protein concentrate made by alcohol precipitation, physical modification with microwave was stu***d.

    为改善醇法大豆浓缩蛋白的功能特性,采用微波技术对醇法大豆浓缩蛋白进行物理改性。

  • The adaptation and technological parameters of ceramic membrane were investigated and compared with the traditional technique of water extraction and alcohol precipitation.

    方法以蝙蝠蛾被毛孢菌丝体水提液为对象,考察陶瓷膜的适配性与工艺参数,并与传统的水提醇沉技术对比。

  • The water-soluble Enteromorpha polysaccharides (EP) were obtained through aqueous extraction-alcohol precipitation technology.

    用热水直接提取、乙醇沉淀的方法提取出浒苔水溶性多糖;

  • Objective: The effect of separation rates on the quality and shelf life of TCM in the process of alcohol precipitation was discussed.

    目的:简要讨论醇沉过程中分离程度对中药质量、保质期等的影响。

  • Objective:To compare the fit and unfit of alcohol precipitation process and chitosan flocculation process used in purification of extract of yanyan buccal tablets.

    目的:比较醇沉法和壳聚糖絮凝法用于咽炎口含片浸膏纯化的优劣。

  • OBJECTIVE To determine the alcohol precipitation techniques for polystictus versicolor polysaccharides.

    目的确定云芝多糖的醇沉工艺。

  • Water extraction with alcohol precipitation was used to prepare for Angelica.

    残渣经水煮、醇沉提取当归多糖;

  • The separation of 1,3-propanediol from fermentative broth by a combination of ultrafiltration and alcohol precipitation was well investigated.

    介绍了超滤-醇沉法从发酵液中提取1,3-*********的工艺。

  • The process conditions of separation and purification via diatom earth filtration and alcohol precipitation were stu***d .

    醇析法是一种有效分离提纯黄原胶的方法。

  • Conclusion:The process of flocculation precipitation is better than the process of alcohol precipitation according to the removal rate of harmful elements and reserved rate of useful elements.

    结论:从有害元素的去除率及有益元素的保留率来看,絮沉工艺优于醇沉工艺。

  • The loss rate of sacidrosiele by using flocculating method is less than that by using alcohol precipitation.

    絮凝法红景天总皂苷损失率明显低于醇沉法,且壳聚糖吸附法的效果最佳。

  • Objective: To study the effects of water extraction, water extract-alcohol precipitation and ethanol ultrasonic extraction on the content of astragalus saponin.

    目的:研究水提法、水提醇沉法、乙醇超生提取法三种提取方法对黄芪总皂苷的影响。

  • Methods Heparin of injection level was treated through degradation by sodium nitrite, desalting by 732 ion exchange resin, neutralization with calcium oxide, separation with alcohol precipitation.

    方法以市售注射用肝素钠为原料 ,经过************降解成低分子肝素钠 ,树脂脱钠 ,氧化钙中和转型成钙盐 ,最后用乙醇沉淀析出。

  • Tannin fractions with different molecular weight distributions were obtained by purification of alcohol precipitation and fractionation of solvent extractions of black wattle bark tannins.

    通过对黑荆树皮单宁进行醇沉纯化和溶剂萃取分级 ,可得到分子量分布范围不同的单宁级分。

  • 专业解析

    酒精沉淀法(Alcohol Precipitation)是一种基于溶液极性变化的分离技术,广泛应用于生物化学和分子生物学领域。该方法通过向溶液中加入乙醇、异丙醇等有机溶剂,降低目标物质(如核酸、多糖或蛋白质)的溶解度,促使其形成固态沉淀物,从而实现纯化或浓缩。

    一、原理与作用机制

    酒精通过改变溶液的介电常数和氢键作用力,破坏溶质分子周围的水合层。例如在DNA纯化中,高浓度乙醇(通常70%-80%)会削弱DNA分子与水分子间的相互作用,促使DNA链间通过磷酸骨架的负电荷与阳离子(如钠离子)结合,聚集成可见沉淀。这一过程符合《分子克隆实验指南》中阐述的电荷屏蔽原理。

    二、核心应用场景

    1. 核酸纯化:75%乙醇常用于去除DNA/RNA提取过程中的蛋白质污染物,美国国立卫生研究院(NIH)实验手册指出该浓度能有效沉淀核酸同时保持其结构完整。
    2. 多糖提取:异丙醇沉淀法可从植物提取液中分离β-葡聚糖,其作用机制被《食品化学》期刊研究证实与多糖分子间的疏水作用增强有关。
    3. 蛋白质浓缩:冷乙醇沉淀法在抗体纯化中具有百年应用历史,通过降低溶液介电常数促使蛋白质疏水区域暴露并聚集。

    三、关键操作参数

    实验效果受三因素显著影响:

    该技术作为基础分离手段,其标准化操作流程已被国际纯粹与应用化学联合会(IUPAC)收录为推荐实验方法。

    网络扩展资料

    “Alcohol precipitation”是一个化学领域术语,结合了“alcohol(醇类)”和“precipitation(沉淀)”的双重含义,指通过加入醇类物质(如乙醇)促使溶液中的特定成分形成沉淀的技术。以下是详细解释:


    1.定义与原理


    2.应用领域


    3.操作流程

    典型的醇沉法(alcohol precipitation procedure)包括以下步骤:

    1. 向水溶液中缓慢加入乙醇,调节浓度至目标范围(如70%-80%)。
    2. 搅拌混合,静置使沉淀完全析出。
    3. 离心或过滤分离沉淀物,去除上清液。
    4. 干燥沉淀以获得纯化产物。

    4.优势与局限


    如需具体案例或扩展应用场景,可参考相关化学实验手册或生物技术文献。

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