[分化] 薄层色谱；[分化] 薄层色谱法；[分化] 薄层层析法
Methods Under the various separating condition, the anticoagulating active components of scorpion were appraised with thin layer chromatography(TLC) and paper chromatography.
方法用不同的展开剂，采用薄层色谱法和纸层析法对全蝎抗凝活性成分进行鉴定和分析。
Objective A rapid, ****** detective method-dot immunogold filtration assay-thin layer chromatography(DIGFA-TLC)for quantitative detecting of Plague was established in this experimental study.
目的建立一种简便、快速的斑点金-薄层色谱法（DIGFA-TLC）用于鼠疫耶尔森氏菌的快速定量诊断。
Thin layer chromatography(TLC) was applied to the separation of oligosaccharides in juice of Jerusalem Artichoke.
采用薄层层析法（TLC）分离菊芋汁中的低聚糖成分。
Objective To discuss the separating condition of the thin layer chromatography(TLC) of the mixture of sudan I and azobenzene.
目的探讨苏丹黄和偶氮苯混合液薄层色谱（TLC）的分离条件。
Methods Polyamide thin layer chromatography is used to determine the spermidine content in urine.
方法采用聚酰胺薄层层析法测定尿液精脒含量。
The method is ******, convenient, rapid and accurate, which is a new try in inspecting thin layer chromatography by ultraviolet.
该方法简单方便，快速准确，结果可靠，是紫外检视薄层色谱的一种新的尝试。
Methods Thin layer chromatography(TLC) was used to qualitatively identify Rhizoma Smilacis Glabrae and Herba Leonuri of Shenfukang capsule.
方法采用薄层色谱法对肾复康胶囊中益母草、土茯苓进行了定性鉴别。
RESULTS: The micro character and thin layer chromatography was clear with no disturbance of negative sample.
结果：显微鉴别特征明显、薄层色普清晰，空白样品无干扰；
Method:TSDP metabolites were detected with thin layer chromatography (TLC) and combination of electrospray ionization mass spectrometry (ESI MS) and sequential tandem mass spectrometry (MS n).
方法：用薄层色谱（TLC）及电喷雾质谱（ESIMS）法检测离体肠内菌对TSDP的代谢产物。
Characterized by thin layer chromatography and fourier transform infrared spectrometry, the biosurfactants producing by TR-1 was identified as one kind of lipopeptide.
经薄层层析、红外光谱鉴定，TR-1菌产生的生物表面活性剂为脂肽类生物表面活性剂。
A quantitative analysis method-Thin layer chromatography-bromination-determine amine method was established for determining two active ingre***nts in the mixture of MTMC and phoxim.
速灭辛乳油是由速灭威和辛硫磷复配而成，采用薄层分离，分别用碱解定胺法和溴化法对两种有效成分进行定量分析。
Identify the conjugated systems of main active elements with UV spectrometry, using thin layer chromatography identify amino in the injection.
并采用紫外分光光度法鉴别其有效成分中的共轭结构，用薄层色谱法鉴别注射液中氨基酸。
Objective To establish a thin layer chromatography scanning method for the determination of cinnamaldehyde content in Qianzheng Plaster.
目的建立牵正膏中桂皮醛含量的薄层扫描测定方法。
Monitoring reactions by thin layer chromatography(TLC).
采用薄层色谱（TLC）监控反应。
METHODS: Use thin layer chromatography, repeated silica gel and RP 18 column chromatography together with spectra as well as synthetic methods.
方法：用薄层色谱，硅胶及反向RP18硅胶柱层析色谱和谱学方法。
The experimental results show that the organic compounds with lower boiling point can not be determined by thin layer chromatography because they are volatile.
结果表明，对于低沸点的化合物，由于样品在点样和展开过程中挥发损失的原因而不能进行显色处理，从而无法进行测定。
The qualitative analysis using thin layer chromatography which crocin-1 and crocin-2 as the check samples and the ethy1 acetate-methanol-water (100:16.
对西红花提取物的精制品进行了分析，定性分析采用薄层色谱法，以西红花甙-1、甙-2为对照品，************-甲醇-水（100：16。
Methods Thin Layer Chromatography (TLC) was used to identify Fructus Lycii and Cortex Moutan.
方法采用薄层层析法（TLC）对枸杞子和牡丹皮进行定性鉴别；
Methods The developers of MAP in Thin Layer Chromatography(TLC)had been decided in the experiments, and the chromatogram condition of MAP with GC/MS had been stu***d.
方法根据*********的特点，用薄层色谱法对*********的展开剂进行了实验选定，对其GC/MS分析的色谱条件进行了探讨。
Organic compounds with various boiling points are determined by thin layer chromatography.
采用薄层层析法对不同沸点的有机化合物进行了测定。
Thin layer chromatography can be a quick method to distinguish the frying oil, hogwash oil from good edible vegetable edible oil.
薄层色谱法可以作为鉴别合格食用植物油与潲水油、煎炸老油的快速方法。
Method:Thin layer chromatography and macroporous resin separation method were used.
方法：薄层层析法及大孔树脂分离方法。
The contents of tyrosine (Tyr) in the ovaries of the mice at various physiological stages were measured by Dansyl chloride reaction and thin layer chromatography on polyamide films.
用薄层层析，荧光光密度法测定了小白鼠卵巢在不同时期及不同生理状态下酪氨酸含量。
Methods Technology of thin layer chromatography(TLC) and high performance liquid chromatography(HPLC).
方法薄层色谱法及高效液相色谱技术。
Results1) The thin layer chromatography could identify the chlorogenic acid and coffee acid in Lonicera fulvotomentosa effectively ;
结果采用薄层色谱法有效鉴别黄褐毛忍冬中的绿原酸、咖啡酸；
Method Asterosaponin was prepared separately by extraction with methanol or ethanol and was analysed by chromogenic reaction , hemolytic test , ultraviolet spectrum and thin layer chromatography.
方法采用乙醇提取法和甲醇提取法从海星体内提取海星总皂苷，用显色反应、溶血实验、紫外光谱分析和薄层色谱对产物进行分析鉴定。
Results:The new standard of thin layer Chromatography spots was clear and the resolution was good.
结果：本制剂新标准薄层图谱斑点清晰，分离度好。
Methods Thin layer chromatography and salt out are adopted to determine the content of esterified catechins.
方法采用盐析的方法对茶多酚粗品液进行处理，薄层色谱扫描仪进行酯型儿茶素含量的测定。
薄层色谱法（Thin Layer Chromatography，TLC）是一种基于吸附原理的分离分析技术，主要用于化学、生物和医药领域中对混合物的快速分离与定性分析。该方法通过在玻璃、铝箔或塑料基板上均匀涂布吸附剂（如硅胶或氧化铝）形成薄层，作为固定相进行物质分离。
工作原理基于不同化合物在固定相（薄层吸附剂）与流动相（展开剂）之间的分配系数差异。当展开剂沿薄层上升时，混合物中的各组分因迁移速率不同而被分离，最终通过显色剂或紫外灯观察斑点位置，常用比移值（Rf值）进行定性分析，计算公式为： $$ R_f = frac{text{组分移动距离}}{text{展开剂移动距离}} $$
典型实验步骤包含四个阶段：（1）制板：将吸附剂浆料均匀涂布于载板并活化；（2）点样：用微量点样器将待测溶液施加于薄层底端；（3）展开：将薄层板置于含展开剂的密闭容器中展开；（4）检测：根据化合物特性选择显色方法。
该方法在药物纯度检测（如《美国药典》收录的抗生素分析）、天然产物分离（如植物生物碱提取）和食品安全检测（如农药残留筛查）中具有广泛应用。相较于柱色谱，其优势在于操作简便、成本低廉且分析速度快，但分离效率低于高效液相色谱法。
权威参考资料：
薄层色谱法（Thin Layer Chromatography，TLC）是一种基于吸附作用的分离分析技术，主要用于快速分离和鉴定混合物中的成分。以下是其核心要点：
1. 基本原理
通过不同物质在固定相（如硅胶或氧化铝薄层）与流动相（有机溶剂）中的分配差异实现分离。极性强的物质更易吸附在固定相上，移动较慢；极性弱的物质则随流动相移动更快。
2. 操作步骤
3. 关键参数（Rf值）
计算迁移率：
$$
R_f = frac{text{斑点移动距离}}{text{溶剂前沿移动距离}}
$$
Rf值具物质特异性，可用于定性分析。
4. 应用领域
5. 优缺点
优点：操作简便、快速（约15-30分钟）、样品量少（微克级）。
缺点：分离效率较低，适合初步分析而非高精度定量。
该技术是实验室常规工具，常与其他色谱法（如HPLC）互补使用。
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