
【医】 Gies' biuret tests
about; annex; canopy; casing; cover; lid; shell; top; build
【化】 cap; cover; lid
【医】 cap; coping; operculum; roof; tegmen; tegmentum; veil
this
【化】 geepound
family name; surname
【医】 biuret test
盖斯氏双缩脲试验(可能为“Gornall双缩脲法”的音译)是一种基于双缩脲反应的蛋白质定量检测方法,其核心原理是通过肽键与铜离子在碱性环境中的显色反应来测定蛋白质含量。以下是详细解释:
双缩脲反应的本质是含有两个或以上肽键(—CONH—)的化合物(如蛋白质或多肽),在碱性条件下与二价铜离子(Cu²⁺)结合,形成紫红色络合物。这种络合物在540nm波长处有最大吸收峰,颜色深浅与蛋白质浓度成正比。
试剂通常包含以下成分:
“盖斯氏”可能源自该方法改良者姓氏的音译(如Gornall)。历史上,Gornall等人于1949年完善了双缩脲法的标准化操作,使其成为实验室常用技术之一。但需注意,中文文献中可能存在音译差异,具体来源需结合原始文献确认。
如需进一步了解试剂配制或实验细节,可参考、4、5中的方法描述。
盖斯氏双缩脲试验(Guthrie Test),是一种新生儿疾病筛查方法。它主要用于检测新生儿是否患有苯丙酮尿症等遗传代谢病。
gài sī shì shuā suǒ shì yàn
The Guthrie Test is a neonatal screening method that is mainly used to detect inherited metabolic disorders such as phenylketonuria.
gʌθri tɛst
The Guthrie Test is typically performed within the first few days of a newborn's life in order to identify any metabolic disorders that may require treatment.
The Guthrie Test is also known as the heel stick test or the PKU test.
There is no direct antonym for the Guthrie Test, as it is simply a screening method and not a specific condition or treatment.
The term "Guthrie Test" is primarily used within the medical field and may not be as commonly known outside of that context.
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