[免疫] 酶联免疫吸附测定，酶标法
Methods The plasma IL-18 in 36 ITP patients and 36 normal controls were detected by enzyme linked immunosorbent assay (ELISA).
方法酶联免疫吸附测定（ELISA）测定血浆IL-18，对36例ITP患者和正常对照组血浆IL-18的水平进行分析。
Plasma OX-LDL level was measured by double antibody enzyme linked immunosorbent assay.
用酶联免疫吸附试验双抗体夹心法检测OX-LDL水平。
Antibody combining sites in an antigenic determinant of cephalosporins were detected by enzyme linked immunosorbent assay (ELISA), hapten inhibition and passive cutaneous anaphylaxis(PCA).
通过ELISA试验、半抗原抑制试验和PCA试验，对头孢菌素抗原决定簇中抗体结合位点进行分析。
The levels of IL-15 in serum was analyzed by enzyme linked immunosorbent assay (ELISA).
使用酶联免疫吸附法（ELISA）测定不同组别血清IL-15水平。
Methods: The levels of IL-12 were measured by enzyme linked immunosorbent assay(ELISA) in 64 patients with viral hepatitis and 20 healthy *****s subject as controls.
方法：双抗体夹心酶联免疫吸附法（ELISA）检测64例病毒性肝炎患者及20例正常对照血清IL-12水平。
OX-LDL of blood plasma was measured by double antibody enzyme linked immunosorbent assay.
用酶联免疫吸附试验双抗体夹心法检测OXLDL。
Objective:To compare the characteristics of the one step and two steps ELISA(Enzyme Linked Immunosorbent Assay) diagnostic kit in detecting of human immunodeficiency virus(HIV) antibo***s.
目的：通过对酶联免疫吸咐试验（ELISA）双抗原夹心一步法和二步法检测人类免疫缺陷病毒（HIV）抗体的比较，探讨各自特点。
Dimer Thrombotic diseases Enzyme linked immunosorbent assay;
二聚体；血栓性疾病；酶联免疫吸附测定；
IL 6 in HECS was measured using sandwich enzyme linked immunosorbent assay(ELISA) method.
采用双抗夹心ELISA法对上清液中IL-6进行定性和定量测定。
The apoptosis rate of lymphocyte cell was determined for workers exposed to high frequency field by biotinavidin enzyme linked immunosorbent assay with enexposed workers as the control group.
采用生物素-亲和素固相酶标法，对在高压工频场强环境中的工作人员进行了淋巴细胞凋亡率的检测，并以非高压作业人员为对照。
Method: 16 patients with MDS and 45 cases of control were subject to the measurement of serum IL-8 levels by sandwich enzyme linked immunosorbent assay (ELISA).
方法：夹心酶联免疫吸附法（ELISA法）检测16例MDS患者血清IL-8，并与正常对照组比较及MDS亚型间比较。
Methods:The serum ACA were tested by enzyme linked immunosorbent assay (ELISA) in 76 patients with cerebral infarction, 42 patients with cerebral hemorrhage and 50 healthy controls.
方法：使用酶联免疫吸附试验 （ELISA），检测76例脑梗死患者，42例脑出血患者以及50名正常人的血清ACA。
Method: 20 healthy human plasma(heparin prevents clotting) and serum levels of the TPS were measured by enzyme linked immunosorbent assay (ELISA);
方法：采用ELISA法测定20名健康人血浆（肝素钠抗凝）和血清TPS水平；
Serum level of Leptinajid prolactin were determined by enzyme linked immunosorbent assay.
用酶联免疫吸附试验（双抗体夹心法）测定血清瘦素、催乳素；
Objective To evaluate the risk of nasopharyngeal carcinoma (NPC) through EB virus antibody profile by enzyme linked immunosorbent assay (ELISA).
目的比较鼻咽癌患者与健康人群血清EB病毒抗体水平，评估患鼻咽癌的危险度。
Enzyme linked immunosorbent assay(ELISA) is think highly of a method, it has many virtues, such as non-radiation pollution, strongly specificity, high sensitivity and timing-shorted.
酶联免疫吸附测定法（ELISA）由于具有无放射性污染、特异性强、灵敏度高、测定周期短、对实验室条件要求不高等许多优点，因而受到人们的普遍关注和重视。
Objectives To establish indirect enzyme linked immunosorbent assay(ELISA)method for detection of human parvovirus B19 antibody and evaluate its importance for clinical application.
目的建立检测人微小病毒B19的间接酶联免疫吸附（ELISA）法，评价其临床应用价值。
The peripheral blood lymphocytes (PBL) were transfected by the mutants and the concentration of SIV core protein P27 in cultured medium was monitored by enzyme linked immunosorbent assay (ELISA).
用突变的病毒转染外周血淋巴细胞（PBL），用ELISA法动态测定培养液中SIV核心蛋白P27浓度。
The titers were determined by enzyme linked immunosorbent assay.
应用酶联免疫法分析半抗原抗体滴度。
Diffusion in gel- enzyme linked immunosorbent assay;
凝胶扩散-酶联免疫吸附试验；
The serum level of IGF-1 by enzyme linked immunosorbent assay (ELISA), the relationships between IGF-1 and the morphology of atherosclerotic plaque in coronary artery were analyzed.
采用酶联免疫吸附法（ELISA）测定其IGF-1水平，分析与冠状动脉斑块形态的关系。
Before the clamp study, Serum MCP-1 was determined by Enzyme Linked ImmunoSorbent Assay(ELISA) and other clinical parameters were determined to analyse the relationship between them.
同时用酶联免疫吸附法（ELISA）测定血清MCP-1水平，并测定其他临床指标，分析各指标之间的相关性及与胰岛素敏感性的关系。
Methods The levels of IL-12 and TNF were measured by enzyme linked immunosorbent assay in 64 patients with viral hepatitis and 20 healthy *****s subject as control.
方法用双抗体夹心酶联免疫吸附法检测64例病毒性肝炎患者及20例正常对照者的血清IL-12与TNF水平。结果病毒性肝炎患者血清IL-12与TNF水平明显高于正常对照组（P。
Methods: G CSF in ascites patients of 25 with SBP and 25 non bacterial infective ascites patients was measured with enzyme linked immunosorbent assay.
方法：应用酶联免疫技术测定25例自发性腹膜炎和25例非细菌感染性腹水患者腹水中的G-CSF。
酶联免疫吸附测定（Enzyme-Linked Immunosorbent Assay，ELISA）是一种基于抗原-抗体特异性结合的高灵敏度生物检测技术，其核心原理是通过酶标记的抗体或抗原与目标分子结合，再利用酶催化底物显色反应实现定量或定性分析。该技术由瑞典科学家Engvall和Perlmann于1971年首次提出，现已成为医学诊断、生物研究和工业检测领域的金标准方法。
ELISA利用固相载体（如96孔板）固定抗原或抗体，通过多步骤反应放大检测信号。其常见类型包括：
根据美国食品药品监督管理局（FDA）批准的检测方案，ELISA在以下领域发挥关键作用：
美国国家生物技术信息中心（NCBI）研究显示，ELISA的检测灵敏度可达pg/mL级别，且具有高通量、可重复性强的特点。但其特异性依赖于抗体质量，可能与其他结构类似物发生交叉反应。当前发展趋向包括与微流控芯片结合实现快速检测，以及采用量子点标记提升多指标联检能力。
酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay，简称ELISA）是一种基于抗原-抗体特异性结合及酶催化反应的高灵敏度免疫学检测技术。以下是其详细解释：
ELISA通过将酶标记在抗体或抗原上，利用固相载体（如96孔微孔板）吸附目标分子，结合洗涤步骤去除未结合物质，最终通过酶促显色反应实现定性或定量分析。其名称可分解为：
通过结合免疫反应的特异性和酶催化的高效性，ELISA成为临床诊断和生物医学研究的重要工具。
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