[免疫] 酶聯免疫吸附測定，酶标法
Methods The plasma IL-18 in 36 ITP patients and 36 normal controls were detected by enzyme linked immunosorbent assay (ELISA).
方法酶聯免疫吸附測定（ELISA）測定血漿IL-18，對36例ITP患者和正常對照組血漿IL-18的水平進行分析。
Plasma OX-LDL level was measured by double antibody enzyme linked immunosorbent assay.
用酶聯免疫吸附試驗雙抗體夾心法檢測OX-LDL水平。
Antibody combining sites in an antigenic determinant of cephalosporins were detected by enzyme linked immunosorbent assay (ELISA), hapten inhibition and passive cutaneous anaphylaxis(PCA).
通過ELISA試驗、半抗原抑制試驗和PCA試驗，對頭孢菌素抗原決定簇中抗體結合位點進行分析。
The levels of IL-15 in serum was analyzed by enzyme linked immunosorbent assay (ELISA).
使用酶聯免疫吸附法（ELISA）測定不同組别血清IL-15水平。
Methods: The levels of IL-12 were measured by enzyme linked immunosorbent assay(ELISA) in 64 patients with viral hepatitis and 20 healthy *****s subject as controls.
方法：雙抗體夾心酶聯免疫吸附法（ELISA）檢測64例病毒性肝炎患者及20例正常對照血清IL-12水平。
OX-LDL of blood plasma was measured by double antibody enzyme linked immunosorbent assay.
用酶聯免疫吸附試驗雙抗體夾心法檢測OXLDL。
Objective:To compare the characteristics of the one step and two steps ELISA(Enzyme Linked Immunosorbent Assay) diagnostic kit in detecting of human immunodeficiency virus(HIV) antibo***s.
目的：通過對酶聯免疫吸咐試驗（ELISA）雙抗原夾心一步法和二步法檢測人類免疫缺陷病毒（HIV）抗體的比較，探讨各自特點。
Dimer Thrombotic diseases Enzyme linked immunosorbent assay;
二聚體；血栓性疾病；酶聯免疫吸附測定；
IL 6 in HECS was measured using sandwich enzyme linked immunosorbent assay(ELISA) method.
采用雙抗夾心ELISA法對上清液中IL-6進行定性和定量測定。
The apoptosis rate of lymphocyte cell was determined for workers exposed to high frequency field by biotinavidin enzyme linked immunosorbent assay with enexposed workers as the control group.
采用生物素-親和素固相酶标法，對在高壓工頻場強環境中的工作人員進行了淋巴細胞凋亡率的檢測，并以非高壓作業人員為對照。
Method: 16 patients with MDS and 45 cases of control were subject to the measurement of serum IL-8 levels by sandwich enzyme linked immunosorbent assay (ELISA).
方法：夾心酶聯免疫吸附法（ELISA法）檢測16例MDS患者血清IL-8，并與正常對照組比較及MDS亞型間比較。
Methods:The serum ACA were tested by enzyme linked immunosorbent assay (ELISA) in 76 patients with cerebral infarction, 42 patients with cerebral hemorrhage and 50 healthy controls.
方法：使用酶聯免疫吸附試驗 （ELISA），檢測76例腦梗死患者，42例腦出血患者以及50名正常人的血清ACA。
Method: 20 healthy human plasma(heparin prevents clotting) and serum levels of the TPS were measured by enzyme linked immunosorbent assay (ELISA);
方法：采用ELISA法測定20名健康人血漿（肝素鈉抗凝）和血清TPS水平；
Serum level of Leptinajid prolactin were determined by enzyme linked immunosorbent assay.
用酶聯免疫吸附試驗（雙抗體夾心法）測定血清瘦素、催乳素；
Objective To evaluate the risk of nasopharyngeal carcinoma (NPC) through EB virus antibody profile by enzyme linked immunosorbent assay (ELISA).
目的比較鼻咽癌患者與健康人群血清EB病毒抗體水平，評估患鼻咽癌的危險度。
Enzyme linked immunosorbent assay(ELISA) is think highly of a method, it has many virtues, such as non-radiation pollution, strongly specificity, high sensitivity and timing-shorted.
酶聯免疫吸附測定法（ELISA）由于具有無放射性污染、特異性強、靈敏度高、測定周期短、對實驗室條件要求不高等許多優點，因而受到人們的普遍關注和重視。
Objectives To establish indirect enzyme linked immunosorbent assay(ELISA)method for detection of human parvovirus B19 antibody and evaluate its importance for clinical application.
目的建立檢測人微小病毒B19的間接酶聯免疫吸附（ELISA）法，評價其臨床應用價值。
The peripheral blood lymphocytes (PBL) were transfected by the mutants and the concentration of SIV core protein P27 in cultured medium was monitored by enzyme linked immunosorbent assay (ELISA).
用突變的病毒轉染外周血淋巴細胞（PBL），用ELISA法動态測定培養液中SIV核心蛋白P27濃度。
The titers were determined by enzyme linked immunosorbent assay.
應用酶聯免疫法分析半抗原抗體滴度。
Diffusion in gel- enzyme linked immunosorbent assay;
凝膠擴散-酶聯免疫吸附試驗；
The serum level of IGF-1 by enzyme linked immunosorbent assay (ELISA), the relationships between IGF-1 and the morphology of atherosclerotic plaque in coronary artery were analyzed.
采用酶聯免疫吸附法（ELISA）測定其IGF-1水平，分析與冠狀動脈斑塊形态的關系。
Before the clamp study, Serum MCP-1 was determined by Enzyme Linked ImmunoSorbent Assay(ELISA) and other clinical parameters were determined to analyse the relationship between them.
同時用酶聯免疫吸附法（ELISA）測定血清MCP-1水平，并測定其他臨床指标，分析各指标之間的相關性及與胰島素敏感性的關系。
Methods The levels of IL-12 and TNF were measured by enzyme linked immunosorbent assay in 64 patients with viral hepatitis and 20 healthy *****s subject as control.
方法用雙抗體夾心酶聯免疫吸附法檢測64例病毒性肝炎患者及20例正常對照者的血清IL-12與TNF水平。結果病毒性肝炎患者血清IL-12與TNF水平明顯高于正常對照組（P。
Methods: G CSF in ascites patients of 25 with SBP and 25 non bacterial infective ascites patients was measured with enzyme linked immunosorbent assay.
方法：應用酶聯免疫技術測定25例自發性腹膜炎和25例非細菌感染性腹水患者腹水中的G-CSF。
酶聯免疫吸附測定（Enzyme-Linked Immunosorbent Assay，ELISA）是一種基于抗原-抗體特異性結合的高靈敏度生物檢測技術，其核心原理是通過酶标記的抗體或抗原與目标分子結合，再利用酶催化底物顯色反應實現定量或定性分析。該技術由瑞典科學家Engvall和Perlmann于1971年首次提出，現已成為醫學診斷、生物研究和工業檢測領域的金标準方法。
ELISA利用固相載體（如96孔闆）固定抗原或抗體，通過多步驟反應放大檢測信號。其常見類型包括：
根據美國食品藥品監督管理局（FDA）批準的檢測方案，ELISA在以下領域發揮關鍵作用：
美國國家生物技術信息中心（NCBI）研究顯示，ELISA的檢測靈敏度可達pg/mL級别，且具有高通量、可重複性強的特點。但其特異性依賴于抗體質量，可能與其他結構類似物發生交叉反應。當前發展趨向包括與微流控芯片結合實現快速檢測，以及采用量子點标記提升多指标聯檢能力。
酶聯免疫吸附試驗（Enzyme-Linked Immunosorbent Assay，簡稱ELISA）是一種基于抗原-抗體特異性結合及酶催化反應的高靈敏度免疫學檢測技術。以下是其詳細解釋：
ELISA通過将酶标記在抗體或抗原上，利用固相載體（如96孔微孔闆）吸附目标分子，結合洗滌步驟去除未結合物質，最終通過酶促顯色反應實現定性或定量分析。其名稱可分解為：
通過結合免疫反應的特異性和酶催化的高效性，ELISA成為臨床診斷和生物醫學研究的重要工具。
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