n. [生化] 疏水作用
Methods: Wash inclusion body effectivity, use Hydrophobic Interaction Chromatography to carry on the purification.
方法：对包涵体进行有效洗涤，采用疏水层析进行纯化。
The hydrophobic interaction force and electrostatic force played a main role in the binding of pentachlorophenol with BSA.
根据所得结果可推断*********与BSA的作用力为疏水作用力和静电作用力。
This proved the adsorption principle was determined by the hydrophobic interaction between surfactants and HAWP, but the strength was affected by the electric charge of each other.
这说明吸附过程的本质由HAWP与表面活性剂之间的疏水效应决定，强度受到两者荷电性质的影响。
Results indicated that hydrophobic interaction and Vader waals forces could be in dominant position in the formation of the complex.
研究结果表明该猝灭为静态猝灭且相应结合作用力以范德华力和疏水作用力为主。
The retention behaviors of aromatic alcohol homologues in hydrophobic interaction chromatography (HIC) were investigated firstly.
首次研究了芳香醇同系物在疏水色谱（HIC）中的保留行为。
The polyethylene glycol (PEG) beaded phase has been employed for biologically active proteins by high performance hydrophobic interaction chromatography.
聚乙二醇键合相已用于高效疏水作用色谱分离活性蛋白质。
The binding of ligands to proteins occurs mostly through weak non-covalent interactions, namely hydrophobic interaction, van der Waals forces, and hydrogen bonding.
蛋白和配体间的作用通常是通过弱非共价键力的相互作用而实现的，如疏水作用、范德华力和氢键。
The interaction between the MIP and target molecules were found to arise from hydrogen bonding in the imprinted area and the hydrophobic interaction in the non-imprinted area .
作为色谱固定相，磺胺甲恶唑烙印聚合物和目标分子之间的相互作用除氢键作用外，也存在着疏水作用。
Hydrogen bond, hydrophobic interaction and electrostatic interaction were major forces in the formation of SPI gels.
氢键、疏水相互作用和静电相互作用是形成大豆分离蛋白凝胶的主要作用力。
Influences of concentration of water in the mobile phase on separation were stu***d, and the results showed that hydrophobic interaction was the major interaction force in molecular recognition.
通过对流动相组成与色谱分离效果关系的探讨，证实溶质与印迹固定相的疏水相互作用是分子识别的主要作用力。
By use of hydrophobic interaction of O, O-diacyl side chain, our research group has first prepared O, O-didodecanoyl chitosan self-assembly vesicles for drug delivery.
本课题组利用O，O—双十二酰壳聚糖侧链的疏水化相互作用，首次成功制备了其自组装药用泡囊。
The results show that hydrophobic interaction plays an important role in polymeric reversed micelle extraction.
研究结果表明，亲油作用在聚合物反胶团萃取过程中起主导作用。
To understand the principle and method of hydrophobic interaction chromatography.
通过实验了解疏水作用层析的原理与方法。
The major bond of conjugation in the formation of complex was probably in the hydrogen bonding and the hydrophobic interaction.
在复合物形成中主要的结合键可能是氢键，以及疏水性作用力。
The retention behaviors of aromatic alcohol homologues in hydrophobic interaction chromatography ( HIC ) were investigated firstly.
首次研究了芳香醇同系物在疏水色谱（HIC ）中的保留行为。
The hydrophobic interaction was the main force according to the thermodynamic parameters of combine for this chrysin derivative with bovine serum albumin.
根据该衍生物和牛血清白蛋白结合的热力学参数，确定了二者之间主要为疏水作用力。
FSA is an assembly process using fluid to transport components to binding sites and the components can orient itself on the binding sites by hydrophobic interaction.
由于流体自我组装元件利用疏水性与亲水性之表面性质差异，驱使具疏水性表面之元件组装于基板上方特定之位置。
First, we have examined the effect of hydrophobic interaction on the adsorption of hydrophobically end-capped poly(ethylene glycol) (HE-PEG) on lipid bilayer.
首先，我们近似定量地研究了疏水作用在高分子对囊泡载体修饰过程中的影响。
The association constants, thermodynamic property and the number of binding sites were obtained. The binding force between tiopronin and BSA was confirmed as hydrophobic interaction.
经研究得到了反应的结合常数、结合热力学性质和结合位点等参数，确定了该药物与BSA之间的作用力类型为疏水作用。
The primary binding pattern between caffeic acid and BSA included electrostatic and hydrophobic interaction, and the latter was identified to be the main force under physiological condition.
二者之间的作用力为静电引力和疏水作用力。在离子强度符合生理条件的情况下，以疏水作用力为主。
The retention mechanism of dencichine on a porous graphitic carbon column seems to be mainly hydrophobic interaction according to the study.
对三七素在石墨化碳柱上的保留及其影响因素的研究结果表明：三七素在石墨化碳柱上的保留机理仍主要是疏水相互作用。
Influences of concentration of water in the mobile phase on separation were stu***d, and the results showed that hydrophobic interaction was the major interaction force in mol…
通过对流动相中水含量与色谱分离效果关系的探讨，结果表明溶质与印迹固定相的疏水作用是分子识别中的主要作用力。
It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interaction chromatography.
进一步提供了可选择的额外的纯化步骤，包括溶胞产物过滤、阴离子交换层 析、三联体亲和层析和疏水性相互作用层析。
For conjugation, immunoglobulin is directly adsorbed on the particle surfaces, mediated mainly by London–van der Waals force and hydrophobic interaction.
对于结合，免疫球蛋白直接吸附在颗粒的表面上，主要通过London-范德华力和疏水相互作用。
There was weak hydrophobic interaction between non-ionic DDAO micelle and DNA.
非离子态DDAO胶束与DNA间存在弱的疏水相互作用。
Hydrogen bonding, electrostatic and hydrophobic interaction are important driving forces for salivary proteins-pigments interaction.
唾液蛋白质与色素的反应驱动力源自氢键结合、静电引力和疏水性作用。
By immobilized metal affinity chromatography, ion-exchange chromatography and hydrophobic interaction chromatography, the recombinant Gln49-PLA2 was purified with 91% purity.
通过固定化金属亲和层析、阴离子交换、疏水层析融合蛋白得到了纯化，其纯度可达91％。
Gel scanning revealed that the purity of human leptin was 42.3%before purification, 89.6%after Q column chromatography and 96.2%after hydrophobic interaction chromatography.
经凝胶扫描分析，纯化前的培养上清液人瘦素纯度为42.3%，Q柱柱层析纯化后纯度达到89.6%，疏水层析进一步纯化后纯度达到96.2%。
The molecular design of conventional amphiphiles of synthetic bilayer membranes was based on the consideration of hydrophobic chain interaction.
合成双分子膜的传统两亲分子设计是基于疏水尾链间相互作用的考虑。
疏水相互作用（hydrophobic interaction）是生物化学和分子生物学中的核心概念，指非极性分子或基团在水环境中因热力学驱动而相互聚集的现象。其本质源于水分子对非极性物质的排斥作用：当疏水性物质进入水相时，水分子会重新排列形成高度有序的“笼状结构”（clathrate structure），导致系统熵值降低。为恢复熵值，疏水基团会自发聚集以减少与水接触的表面积。
这一现象的分子机制可通过热力学公式解释： $$ Delta G = Delta H - TDelta S $$ 当疏水分子聚集时，水分子从有序状态转变为自由状态，系统的熵增（$Delta S$正值）成为主要驱动力，最终使吉布斯自由能（$Delta G$）降低。
在蛋白质折叠过程中，疏水相互作用起关键作用。例如肌红蛋白的核心区域常包含疏水性氨基酸（如亮氨酸、异亮氨酸），这些残基通过疏水效应形成稳定的三维结构。该原理同样应用于药物设计领域，脂溶性药物分子可通过疏水作用与靶标蛋白结合。
权威研究证实，疏水作用强度随温度升高而增强，这与离子键等作用力的温度依赖性形成鲜明对比。这种特性在热稳定酶的进化过程中具有重要生物学意义（Nature子刊《Nature Structural & Molecular Biology》第28卷第3期）。
参考资料
Hydrophobic interaction（疏水相互作用） 是指非极性分子或分子中的疏水基团在水环境中倾向于聚集以减少与水接触的现象。这种作用并非直接的化学键，而是由热力学驱动，主要源于水分子对疏水物质的排斥效应。
原理
当疏水物质（如脂类、蛋白质的非极性侧链）进入水中时，水分子会围绕其形成高度有序的“笼状”结构（称为水合层），导致系统熵降低。为恢复熵值，疏水物质倾向于聚集，减少与水的接触面积，从而形成疏水相互作用。
生物学意义
应用
示例：油滴在水中聚集成大油滴，即为疏水相互作用的直观体现。
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