n. [生化] 疏水作用
Methods: Wash inclusion body effectivity, use Hydrophobic Interaction Chromatography to carry on the purification.
方法：對包涵體進行有效洗滌，采用疏水層析進行純化。
The hydrophobic interaction force and electrostatic force played a main role in the binding of pentachlorophenol with BSA.
根據所得結果可推斷*********與BSA的作用力為疏水作用力和靜電作用力。
This proved the adsorption principle was determined by the hydrophobic interaction between surfactants and HAWP, but the strength was affected by the electric charge of each other.
這說明吸附過程的本質由HAWP與表面活性劑之間的疏水效應決定，強度受到兩者荷電性質的影響。
Results indicated that hydrophobic interaction and Vader waals forces could be in dominant position in the formation of the complex.
研究結果表明該猝滅為靜态猝滅且相應結合作用力以範德華力和疏水作用力為主。
The retention behaviors of aromatic alcohol homologues in hydrophobic interaction chromatography (HIC) were investigated firstly.
首次研究了芳香醇同系物在疏水色譜（HIC）中的保留行為。
The polyethylene glycol (PEG) beaded phase has been employed for biologically active proteins by high performance hydrophobic interaction chromatography.
聚乙二醇鍵合相已用于高效疏水作用色譜分離活性蛋白質。
The binding of ligands to proteins occurs mostly through weak non-covalent interactions, namely hydrophobic interaction, van der Waals forces, and hydrogen bonding.
蛋白和配體間的作用通常是通過弱非共價鍵力的相互作用而實現的，如疏水作用、範德華力和氫鍵。
The interaction between the MIP and target molecules were found to arise from hydrogen bonding in the imprinted area and the hydrophobic interaction in the non-imprinted area .
作為色譜固定相，磺胺甲惡唑烙印聚合物和目标分子之間的相互作用除氫鍵作用外，也存在着疏水作用。
Hydrogen bond, hydrophobic interaction and electrostatic interaction were major forces in the formation of SPI gels.
氫鍵、疏水相互作用和靜電相互作用是形成大豆分離蛋白凝膠的主要作用力。
Influences of concentration of water in the mobile phase on separation were stu***d, and the results showed that hydrophobic interaction was the major interaction force in molecular recognition.
通過對流動相組成與色譜分離效果關系的探讨，證實溶質與印迹固定相的疏水相互作用是分子識别的主要作用力。
By use of hydrophobic interaction of O, O-diacyl side chain, our research group has first prepared O, O-didodecanoyl chitosan self-assembly vesicles for drug delivery.
本課題組利用O，O—雙十二酰殼聚糖側鍊的疏水化相互作用，首次成功制備了其自組裝藥用泡囊。
The results show that hydrophobic interaction plays an important role in polymeric reversed micelle extraction.
研究結果表明，親油作用在聚合物反膠團萃取過程中起主導作用。
To understand the principle and method of hydrophobic interaction chromatography.
通過實驗了解疏水作用層析的原理與方法。
The major bond of conjugation in the formation of complex was probably in the hydrogen bonding and the hydrophobic interaction.
在複合物形成中主要的結合鍵可能是氫鍵，以及疏水性作用力。
The retention behaviors of aromatic alcohol homologues in hydrophobic interaction chromatography ( HIC ) were investigated firstly.
首次研究了芳香醇同系物在疏水色譜（HIC ）中的保留行為。
The hydrophobic interaction was the main force according to the thermodynamic parameters of combine for this chrysin derivative with bovine serum albumin.
根據該衍生物和牛血清白蛋白結合的熱力學參數，确定了二者之間主要為疏水作用力。
FSA is an assembly process using fluid to transport components to binding sites and the components can orient itself on the binding sites by hydrophobic interaction.
由于流體自我組裝元件利用疏水性與親水性之表面性質差異，驅使具疏水性表面之元件組裝于基闆上方特定之位置。
First, we have examined the effect of hydrophobic interaction on the adsorption of hydrophobically end-capped poly(ethylene glycol) (HE-PEG) on lipid bilayer.
首先，我們近似定量地研究了疏水作用在高分子對囊泡載體修飾過程中的影響。
The association constants, thermodynamic property and the number of binding sites were obtained. The binding force between tiopronin and BSA was confirmed as hydrophobic interaction.
經研究得到了反應的結合常數、結合熱力學性質和結合位點等參數，确定了該藥物與BSA之間的作用力類型為疏水作用。
The primary binding pattern between caffeic acid and BSA included electrostatic and hydrophobic interaction, and the latter was identified to be the main force under physiological condition.
二者之間的作用力為靜電引力和疏水作用力。在離子強度符合生理條件的情況下，以疏水作用力為主。
The retention mechanism of dencichine on a porous graphitic carbon column seems to be mainly hydrophobic interaction according to the study.
對三七素在石墨化碳柱上的保留及其影響因素的研究結果表明：三七素在石墨化碳柱上的保留機理仍主要是疏水相互作用。
Influences of concentration of water in the mobile phase on separation were stu***d, and the results showed that hydrophobic interaction was the major interaction force in mol…
通過對流動相中水含量與色譜分離效果關系的探讨，結果表明溶質與印迹固定相的疏水作用是分子識别中的主要作用力。
It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interaction chromatography.
進一步提供了可選擇的額外的純化步驟，包括溶胞産物過濾、陰離子交換層 析、三聯體親和層析和疏水性相互作用層析。
For conjugation, immunoglobulin is directly adsorbed on the particle surfaces, mediated mainly by London–van der Waals force and hydrophobic interaction.
對于結合，免疫球蛋白直接吸附在顆粒的表面上，主要通過London-範德華力和疏水相互作用。
There was weak hydrophobic interaction between non-ionic DDAO micelle and DNA.
非離子态DDAO膠束與DNA間存在弱的疏水相互作用。
Hydrogen bonding, electrostatic and hydrophobic interaction are important driving forces for salivary proteins-pigments interaction.
唾液蛋白質與色素的反應驅動力源自氫鍵結合、靜電引力和疏水性作用。
By immobilized metal affinity chromatography, ion-exchange chromatography and hydrophobic interaction chromatography, the recombinant Gln49-PLA2 was purified with 91% purity.
通過固定化金屬親和層析、陰離子交換、疏水層析融合蛋白得到了純化，其純度可達91％。
Gel scanning revealed that the purity of human leptin was 42.3%before purification, 89.6%after Q column chromatography and 96.2%after hydrophobic interaction chromatography.
經凝膠掃描分析，純化前的培養上清液人瘦素純度為42.3%，Q柱柱層析純化後純度達到89.6%，疏水層析進一步純化後純度達到96.2%。
The molecular design of conventional amphiphiles of synthetic bilayer membranes was based on the consideration of hydrophobic chain interaction.
合成雙分子膜的傳統兩親分子設計是基于疏水尾鍊間相互作用的考慮。
疏水相互作用（hydrophobic interaction）是生物化學和分子生物學中的核心概念，指非極性分子或基團在水環境中因熱力學驅動而相互聚集的現象。其本質源于水分子對非極性物質的排斥作用：當疏水性物質進入水相時，水分子會重新排列形成高度有序的“籠狀結構”（clathrate structure），導緻系統熵值降低。為恢複熵值，疏水基團會自發聚集以減少與水接觸的表面積。
這一現象的分子機制可通過熱力學公式解釋： $$ Delta G = Delta H - TDelta S $$ 當疏水分子聚集時，水分子從有序狀态轉變為自由狀态，系統的熵增（$Delta S$正值）成為主要驅動力，最終使吉布斯自由能（$Delta G$）降低。
在蛋白質折疊過程中，疏水相互作用起關鍵作用。例如肌紅蛋白的核心區域常包含疏水性氨基酸（如亮氨酸、異亮氨酸），這些殘基通過疏水效應形成穩定的三維結構。該原理同樣應用于藥物設計領域，脂溶性藥物分子可通過疏水作用與靶标蛋白結合。
權威研究證實，疏水作用強度隨溫度升高而增強，這與離子鍵等作用力的溫度依賴性形成鮮明對比。這種特性在熱穩定酶的進化過程中具有重要生物學意義（Nature子刊《Nature Structural & Molecular Biology》第28卷第3期）。
參考資料
Hydrophobic interaction（疏水相互作用） 是指非極性分子或分子中的疏水基團在水環境中傾向于聚集以減少與水接觸的現象。這種作用并非直接的化學鍵，而是由熱力學驅動，主要源于水分子對疏水物質的排斥效應。
原理
當疏水物質（如脂類、蛋白質的非極性側鍊）進入水中時，水分子會圍繞其形成高度有序的“籠狀”結構（稱為水合層），導緻系統熵降低。為恢複熵值，疏水物質傾向于聚集，減少與水的接觸面積，從而形成疏水相互作用。
生物學意義
應用
示例：油滴在水中聚集成大油滴，即為疏水相互作用的直觀體現。
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