[分化] 排阻色谱法；筛析色谱法
Investigations on protein refolding by size-exclusion chromatography (SEC) was carried out by using lysozyme as a model system.
以溶菌酶为模拟体系对体积排阻色谱法进行蛋白质折叠过程实验研究。
A general method for determining the composition of the eopolymeriatkm products by quantitative size exclusion chromatography (SEC ) is proposed.
本文给出了一种测定共聚产物组成的一般性体积排除色谱定量方法。
The chain length distribution of a oligomeric polystyrene sample initiated by n-butyl lithium was determined by high performance size exclusion chromatography on a ultraviolet absorption detector.
用高效尺寸排除色谱以紫外吸收光度计寿检测器，测定了一个以*********锂引发的聚*********齐聚物的链长分布。
Among all the methods, size exclusion chromatography attracts more attention for its high yield and convenient operation.
在层析技术中分子排阻色谱法由于操作简便 ，复性效率高 ，成为研究的重点。
The content of benzoic acid in beverages was determined by ion-exclusion chromatography.
应用离子排斥色谱法测定了饮料中的苯甲酸含量。
The analytical results from size exclusion chromatography confirmed that more large peptides with higher molecular weight were generated in the modified products.
排阻色谱分析结果确认，修饰产物中有更多的高分子质量肽段存在。
Degradation of lyophilized formulations was quantified using size exclusion (SEC) and ion-exchange chromatography (IEX).
冻干制剂降解是量化使用尺寸排阻（SEC ）的和离子交换层析（IEX ） 。
The molecular exclusion chromatography(MEC') was used in determination of protein purity.
用分子排阻色谱法测定其蛋白纯度。
METHOD:Reversed phase high performance liquid chromatography(RP HPLC) and high performace size exclusion chromatography(SEC) were used.
方法：反相高效液相色谱（RPHPLC）法及高效体积排阻色谱法（SEC）。
Determination of organic acids in soy by Ion-exclusion chromatography with direct conductivity detection was developed.
研究了直接电导检测离子排斥色谱法分离测定酱油中多种有机酸。
Size-exclusion chromatography, as a special liquid chromatography, is used to determine the molecular weight distribution and preparatively separate the samples according to the molecular weight.
排阻色谱法做为一种特殊的液相色谱技术可用于分子量分布的测定及按分子量的不同进行的制备分离。
Methods Several items of hepatolysate solution extracted from pig and cattle were tested based on standards, and using size-exclusion chromatography the peptide mappings were analyzed.
方法分别选取猪源、牛源肝水解肽溶液，依据标准检测部分项目，并用分子排阻色谱法进行肽图分析。
An ion-exclusion chromatography method for simultaneous determination of mainly Metabolites and glucose in fumaric acid fermentation broth of Rhizopus Oryzae was developed.
提出了利用离子排斥色谱法同时分析米根霉富马酸发酵液中主要代谢产物与葡萄糖的方法。
The results of overall process, size exclusion chromatography and the model of describing particle partition coefficient of cellulose gel are found.
得到了他们的孔隙率、排斥分离尺寸和描述微粒分配平衡系数的模型。
The procedure and results of quantitative determination of partially hydrolyzed polyacrylamide (PHPA) in chemical flooding systems by size exclusion chromatography are described.
介绍了用分子排阻色谱技术定量测定化学驱油体系中的部分水解聚************（PHPA）的方法。
The conditions of ion exclusion chromatography was applied to the determination of boric acid in soil and plants were explored with tartaric acid- mannitol as eluent and NaOH as regenerant.
本文研究了用酒石酸-甘露醇作淋洗液，************作再生液测定土壤和植物中硼酸的离子排斥色谱条件。
AIM: To study the application of urea gra***nt size exclusion chromatography (SEC) in the renaturation with simultaneous purification of recombinant human stem cell factor (rhSCF) expressed in E.
目的：用脲梯度体积排阻色谱（SEC）复性并同时纯化在大肠杆菌中高表达的人干细胞因子。
The molecular weight distribution and content of organics in membrane bioreactor were analyzed with the combination of steric exclusion chromatography and refractive index detector.
本研究将空间排阻液相色谱和示差折光检测器联用，测定膜生物反应器中有机物的分子量分布特性并进行定量分析。
The relative molecular mass of permeates was investigated by high performance size exclusion chromatography.
利用高效凝胶排阻色谱技术分析超滤滤过液的相对分子量分布。
A method is presented for measuring the composition and molecular weight of block copolymer simultaneously by size exclusion chromatography with a single detector.
本文建议了一种使用具有单一检测器的常规尺寸排除色谱仪同时测量嵌段共聚物纽成和分子量的方法。
SEC2 was separated and purified successively by ultrafiltration, salting out with ammonium sulfate, ion- exchange chromatography and molecular exclusion chromatography.
发酵液经超滤浓缩、硫酸铵沉淀、阴离子交换色谱和分子筛色谱，获得了纯度为90%的SEC2，总收率23%。
Ion-exclusion chromatography is an effective method for analysis of organic acids.
离子排斥色谱法（IEC）是分析有机酸的有效方法。
排阻色谱法（Exclusion Chromatography），又称凝胶色谱法（Gel Chromatography）或尺寸排阻色谱法（Size Exclusion Chromatography, SEC），是一种基于溶质分子尺寸（流体力学体积）差异进行分离的液相色谱技术。
其核心分离原理在于色谱柱内填充的多孔固定相（凝胶或树脂）。当含有不同大小分子的混合物溶液流经色谱柱时：
因此，溶质分子按照从大到小的顺序依次被洗脱分离。其分离过程主要基于分子在孔隙中的空间排阻效应，而非分子与固定相之间的化学相互作用（如吸附、分配、离子交换等）。
主要特点与应用：
权威性参考来源：
“Exclusion chromatography”（排阻色谱法）是一种基于分子尺寸差异进行分离的色谱技术，广泛应用于高分子和生物大分子的分析。以下是详细解释：
该方法利用多孔填料（如多孔硅胶或树脂）的孔径大小差异分离物质。当样品溶液流经色谱柱时，大分子因无法进入填料孔隙而快速通过（体积排阻效应），小分子则渗入孔隙中导致保留时间延长。
以上信息综合了多个权威来源，若需更详细原理或实验方法，可参考色谱分析专业文献。
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