[分化] 排阻色譜法；篩析色譜法
Investigations on protein refolding by size-exclusion chromatography (SEC) was carried out by using lysozyme as a model system.
以溶菌酶為模拟體系對體積排阻色譜法進行蛋白質折疊過程實驗研究。
A general method for determining the composition of the eopolymeriatkm products by quantitative size exclusion chromatography (SEC ) is proposed.
本文給出了一種測定共聚産物組成的一般性體積排除色譜定量方法。
The chain length distribution of a oligomeric polystyrene sample initiated by n-butyl lithium was determined by high performance size exclusion chromatography on a ultraviolet absorption detector.
用高效尺寸排除色譜以紫外吸收光度計壽檢測器，測定了一個以*********锂引發的聚*********齊聚物的鍊長分布。
Among all the methods, size exclusion chromatography attracts more attention for its high yield and convenient operation.
在層析技術中分子排阻色譜法由于操作簡便 ，複性效率高 ，成為研究的重點。
The content of benzoic acid in beverages was determined by ion-exclusion chromatography.
應用離子排斥色譜法測定了飲料中的苯甲酸含量。
The analytical results from size exclusion chromatography confirmed that more large peptides with higher molecular weight were generated in the modified products.
排阻色譜分析結果确認，修飾産物中有更多的高分子質量肽段存在。
Degradation of lyophilized formulations was quantified using size exclusion (SEC) and ion-exchange chromatography (IEX).
凍幹制劑降解是量化使用尺寸排阻（SEC ）的和離子交換層析（IEX ） 。
The molecular exclusion chromatography(MEC') was used in determination of protein purity.
用分子排阻色譜法測定其蛋白純度。
METHOD:Reversed phase high performance liquid chromatography(RP HPLC) and high performace size exclusion chromatography(SEC) were used.
方法：反相高效液相色譜（RPHPLC）法及高效體積排阻色譜法（SEC）。
Determination of organic acids in soy by Ion-exclusion chromatography with direct conductivity detection was developed.
研究了直接電導檢測離子排斥色譜法分離測定醬油中多種有機酸。
Size-exclusion chromatography, as a special liquid chromatography, is used to determine the molecular weight distribution and preparatively separate the samples according to the molecular weight.
排阻色譜法做為一種特殊的液相色譜技術可用于分子量分布的測定及按分子量的不同進行的制備分離。
Methods Several items of hepatolysate solution extracted from pig and cattle were tested based on standards, and using size-exclusion chromatography the peptide mappings were analyzed.
方法分别選取豬源、牛源肝水解肽溶液，依據标準檢測部分項目，并用分子排阻色譜法進行肽圖分析。
An ion-exclusion chromatography method for simultaneous determination of mainly Metabolites and glucose in fumaric acid fermentation broth of Rhizopus Oryzae was developed.
提出了利用離子排斥色譜法同時分析米根黴富馬酸發酵液中主要代謝産物與葡萄糖的方法。
The results of overall process, size exclusion chromatography and the model of describing particle partition coefficient of cellulose gel are found.
得到了他們的孔隙率、排斥分離尺寸和描述微粒分配平衡系數的模型。
The procedure and results of quantitative determination of partially hydrolyzed polyacrylamide (PHPA) in chemical flooding systems by size exclusion chromatography are described.
介紹了用分子排阻色譜技術定量測定化學驅油體系中的部分水解聚************（PHPA）的方法。
The conditions of ion exclusion chromatography was applied to the determination of boric acid in soil and plants were explored with tartaric acid- mannitol as eluent and NaOH as regenerant.
本文研究了用酒石酸-甘露醇作淋洗液，************作再生液測定土壤和植物中硼酸的離子排斥色譜條件。
AIM: To study the application of urea gra***nt size exclusion chromatography (SEC) in the renaturation with simultaneous purification of recombinant human stem cell factor (rhSCF) expressed in E.
目的：用脲梯度體積排阻色譜（SEC）複性并同時純化在大腸杆菌中高表達的人幹細胞因子。
The molecular weight distribution and content of organics in membrane bioreactor were analyzed with the combination of steric exclusion chromatography and refractive index detector.
本研究将空間排阻液相色譜和示差折光檢測器聯用，測定膜生物反應器中有機物的分子量分布特性并進行定量分析。
The relative molecular mass of permeates was investigated by high performance size exclusion chromatography.
利用高效凝膠排阻色譜技術分析超濾濾過液的相對分子量分布。
A method is presented for measuring the composition and molecular weight of block copolymer simultaneously by size exclusion chromatography with a single detector.
本文建議了一種使用具有單一檢測器的常規尺寸排除色譜儀同時測量嵌段共聚物紐成和分子量的方法。
SEC2 was separated and purified successively by ultrafiltration, salting out with ammonium sulfate, ion- exchange chromatography and molecular exclusion chromatography.
發酵液經超濾濃縮、硫酸铵沉澱、陰離子交換色譜和分子篩色譜，獲得了純度為90%的SEC2，總收率23%。
Ion-exclusion chromatography is an effective method for analysis of organic acids.
離子排斥色譜法（IEC）是分析有機酸的有效方法。
排阻色譜法（Exclusion Chromatography），又稱凝膠色譜法（Gel Chromatography）或尺寸排阻色譜法（Size Exclusion Chromatography, SEC），是一種基于溶質分子尺寸（流體力學體積）差異進行分離的液相色譜技術。
其核心分離原理在于色譜柱内填充的多孔固定相（凝膠或樹脂）。當含有不同大小分子的混合物溶液流經色譜柱時：
因此，溶質分子按照從大到小的順序依次被洗脫分離。其分離過程主要基于分子在孔隙中的空間排阻效應，而非分子與固定相之間的化學相互作用（如吸附、分配、離子交換等）。
主要特點與應用：
權威性參考來源：
“Exclusion chromatography”（排阻色譜法）是一種基于分子尺寸差異進行分離的色譜技術，廣泛應用于高分子和生物大分子的分析。以下是詳細解釋：
該方法利用多孔填料（如多孔矽膠或樹脂）的孔徑大小差異分離物質。當樣品溶液流經色譜柱時，大分子因無法進入填料孔隙而快速通過（體積排阻效應），小分子則滲入孔隙中導緻保留時間延長。
以上信息綜合了多個權威來源，若需更詳細原理或實驗方法，可參考色譜分析專業文獻。
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