
abbr. 细胞培养基(dulbecco's modified eagle medium)
Serum-free DMEM medium with high glucose works for us.
我们使用的是不含血清的高糖DMEM培养基,效果很好。
Objective To compare the culture effects of CHO-C28 cells by domestic and imported DMEM.
目的比较国产与进口DMEM培养基培养cho - C28细胞的效果。
The isolated cells were cultured in DMEM medium and PDL fibroblasts were purified in subculture.
在D MEM培养基中进行了原代及传代培养,对其中的成纤维细胞进行分离纯化。
The cells were planked with lower cell density, and further screened in DMEM medium containing G418 MFD;
采用较低的细胞密度铺板,加入含最小致死量G418的DMEM培养基进行再次筛选;
The friction force of PGA porous scaffolds between DMEM is increases with the increase of the flow velocity.
聚羟基乙酸PGA管状支架的摩擦力随流速的增加而增加。
DMEM(Dulbecco's Modified Eagle's Medium)是一种广泛应用于生物医学研究的细胞培养基,由科学家Renato Dulbecco在Eagle's基础培养基(BME)基础上改良而成。其主要特点包括:
组成与功能
该培养基包含高浓度葡萄糖(4500 mg/L)、氨基酸(如L-谷氨酰胺)、维生素、无机盐及缓冲体系(如碳酸氢钠),可支持哺乳动物细胞(如HEK293、HeLa细胞)的高密度生长。与基础培养基相比,DMEM的营养成分浓度提升至2-4倍,适用于快速增殖的细胞类型。
应用领域
DMEM被用于病毒培养(如腺病毒载体生产)、单克隆抗体开发、干细胞分化实验及癌症研究。例如在新冠疫苗研发中,DMEM被用于培养表达病毒刺突蛋白的细胞系。
配方变体
根据实验需求存在多种改良版本,包括:
参考文献
DMEM(全称Dulbecco's Modified Eagle Medium)是生物医学领域常用的细胞培养基,其名称来源于开发者杜尔贝科(Dulbecco)对基础Eagle培养基(Eagle's Minimum Essential Medium, MEM)的改良。以下是详细解释:
如需更完整的配方或应用案例,的权威来源。
sea levelturn one's back totake in one's strideconfronteddazzlesdispatchingeducationallymagnetoresistanceMidasPunjabiquillrecreationalsanctimoniousnessStalintenementsair rifleat breakfasthorizontal mergerimport substitutionpainted steelproprietary technologytrace amountaglimmeralexandritearythmiaatropaminedemesnedemodulateincombustibleMaypole