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agarose gel electrophoresis是什么意思,agarose gel electrophoresis的意思翻译、用法、同义词、例句

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  • 琼脂糖凝胶电泳

  • 例句

  • Conclusion It is good application prospects to determine the LDH isoenzyme by improvement agarose gel electrophoresis method.

    结论改良琼脂糖凝胶电泳法测定血清LDH同工酶应用前景良好。

  • Methods PCR reaction systems of RAPD and ISSR were optimized, and the agarose gel electrophoresis results were analyzed into statistic data.

    方法优化RAPD及ISSR的PCR反应体系,对产物琼脂糖凝胶电泳结果进行数据统计。

  • Methods The length of genetic segment of SCA1, SCA2 and SCA3 in 92 patients with ataxia of unknown origin were detected by polymerase chain reaction (PCR) and agarose gel electrophoresis .

    方法应用PCR方法和琼脂糖凝胶电泳对92例不明原因共济失调先证患者测定其SCA1、SCA2和SCA3基因片段长度。

  • The characteristic DNA ladder was found in the agarose gel electrophoresis of the nucleus DNA of tranfsected cells.

    细胞核DNA琼脂糖凝胶电泳呈现凋亡细胞特有的梯状条带;

  • Amplified IPCR products were evaluated by 1.8% agarose gel electrophoresis and ethidium bromide staining.

    扩增产物在1-8 % 的琼脂糖凝胶上进行电泳分离,溴化乙锭染色。

  • A ladder-like pattern of DNA fragmentation appeared upon agarose gel electrophoresis.

    琼脂糖凝胶电泳可见“梯状”现象;

  • DNA agarose gel electrophoresis revealed nuclear fragmentation (DNA ladder).

    DNA琼脂糖凝胶电泳显示典型的梯状条带。

  • Agarose gel electrophoresis showed evident DNA fragmentation.

    DNA琼脂糖凝胶电泳可见明显的梯状条带;

  • Methods:Starch-agarose gel electrophoresis is used to observe the interaction of human Hb on rat and mouse Hb.

    方法:采用淀粉-琼脂糖混合凝胶电泳对人及大小鼠血红蛋白成分分别进行互作实验,观察其电泳行为进行判断。

  • The result of agarose gel electrophoresis illustrated that it was a better method to separate nucleic acid from cell suspension of histiocyte and blood.

    电泳结果表明磁性微球可以很好地从细胞悬液、组织、血液等样品中分离得到高质量的核酸。

  • Objective To analyse the clinical feasibility of two electrophoresis procedures of seminal plasma proteins, agarose gel electrophoresis and SDS-agarose gel electrophoresis.

    目的探讨琼脂糖电泳和SDS-琼脂糖电泳分析精浆蛋白质的临床可行性。

  • Methods YAP element was detected by PCR amplification and agarose gel electrophoresis.

    方法利用PCR法对此位点进行扩增,经过琼脂糖凝胶电泳检测结果。

  • Agarose gel electrophoresis could be of value in rapid screening and diagnosis of rotavirus infection as a sensitive, specific, and inexpensive test.

    可作为一种快速方法用于轮状病毒感染的筛选和快速诊断。

  • DNA ladder was detected by agarose gel electrophoresis.

    琼脂糖凝胶电泳检测DNA梯状条带。

  • Method:The DNA gradent bands were detected by agarose gel electrophoresis.

    方法:用琼脂糖凝胶电泳法观察DNA的梯状条带。

  • The level of purified superhelix plasmids were measured by 1% agarose gel electrophoresis.

    所得纯化物以1%琼脂糖凝胶电泳鉴定超螺旋质粒的含量。

  • Fluorescence staining and agarose gel electrophoresis were used to study apoptosis.

    用荧光染色法和琼脂糖凝胶电泳观察细胞凋亡情况。

  • DNA ladder was showed by agarose gel electrophoresis.

    琼脂糖凝胶电泳测定DNA梯状带。

  • Methods:DNA damage was measured by the loss of fluorescence after DNA binding to ethidium bromide and by the increased DNA mobility in agarose gel electrophoresis.

    方法:通过DNA结合溴乙锭后的荧光损失和DNA琼脂糖凝胶电泳的迁移率来测定DNA的损伤程度。

  • PCR amplification and agarose gel electrophoresis.

    PCR扩增和琼脂糖凝胶电泳分析。

  • Amplification products were analyzed by agarose gel electrophoresis.

    扩增产物通过琼脂糖凝胶电泳检测和分析。

  • Agarose gel electrophoresis was used to analyze DNA character and the flow cytometry to detect apoptotic rate and cell cycle distribution.

    琼脂糖凝胶电泳分析DNA特征;流式细胞仪检测细胞凋亡和细胞周期分布;

  • Methods: Apoptosis was detected with quantitation of DNA fragmentation, agarose gel electrophoresis of DNA ladder, flowcytometric analysis and morphological examination of apoptotic cells.

    方法:用DNA电泳、DNA片段定量测定技术、流式细胞术分析及光镜和电镜观察凋亡细胞。

  • The DNA fragments were detected through agarose gel electrophoresis.

    琼脂糖凝胶电泳检测DNA的碎片;

  • Methods:By checking the morphology of apoptotic cells, agarose gel electrophoresis of DNA fragmentation and quantitation of apoptotic cells by means of flow cytometric analysis.

    方法:应用细胞形态学检查,DNA凝胶电泳及流式细胞仪分析检测。

  • 专业解析

    琼脂糖凝胶电泳(Agarose Gel Electrophoresis)是分子生物学和生物化学中用于分离、鉴定和纯化DNA或RNA片段的一项基础且关键的技术。其核心原理是利用电场驱动带电生物大分子(主要是核酸)通过琼脂糖凝胶基质,依据分子大小(长度)进行分离。以下是详细解释:

    一、 定义与基本原理

    二、 关键组分与材料

    1. 琼脂糖: 不同浓度的琼脂糖凝胶适用于分离不同大小范围的DNA片段。浓度越高,凝胶孔径越小,适合分离小片段(如0.5-2%用于0.1-3 kb的DNA);浓度越低,孔径越大,适合分离大片段(如0.3-0.7%用于5-60 kb的DNA)。
    2. 电泳缓冲液:
      • 常用缓冲液: TAE (Tris-乙酸-EDTA) 和 TBE (Tris-硼酸-EDTA)。它们维持稳定的pH值和离子强度,提供电泳所需的导电性。
      • 作用: 维持核酸稳定性(EDTA螯合Mg²⁺抑制DNase)、提供导电离子、维持pH稳定(Tris)。
    3. 核酸染料:
      • 目的: 使原本无色的核酸条带在特定光源下可见。
      • 常用染料:
        • 溴化乙锭(Ethidium Bromide, EtBr): 传统染料,插入DNA双链发出橙色荧光(紫外光激发)。因有潜在致突变性,使用时需谨慎。
        • SYBR系列染料(如SYBR Safe, SYBR Gold): 更安全的替代品,灵敏度高,毒性低,部分可用蓝光激发。
      • 使用方式: 可加入凝胶和缓冲液中(预染),或在电泳后将凝胶浸入染料溶液中染色(后染)。
    4. DNA分子量标准(DNA Ladder): 包含一系列已知大小的DNA片段,与样品一同电泳,用于确定样品DNA片段的大小(通过比较迁移距离)。
    5. 电源和电泳槽: 提供稳定的直流电场。

    三、 标准操作流程

    1. 制备凝胶: 称取适量琼脂糖粉末,加入电泳缓冲液,加热煮沸溶解。冷却至约60°C后,加入核酸染料(如选择预染),倒入制胶模具中,插入梳子。冷却凝固后形成带有上样孔的凝胶。
    2. 准备样品: 将DNA或RNA样品与上样缓冲液混合。上样缓冲液通常含甘油(增加样品密度使其沉入孔底)和示踪染料(如溴酚蓝、二甲苯青,用于监测电泳进程)。
    3. 上样: 将凝胶放入装有电泳缓冲液的电泳槽中(缓冲液需没过凝胶表面)。小心将样品混合物加入上样孔中。在其中一个孔中加入DNA分子量标准。
    4. 电泳: 连接电源(负极靠近上样孔,正极在另一端),设置合适的电压(通常1-10 V/cm凝胶长度)。电泳时间取决于凝胶浓度、电压和待分离片段大小。
    5. 成像: 电泳结束后,取出凝胶。如果使用后染法,需将凝胶在染色液中浸泡一定时间,可能还需要脱色。将凝胶置于紫外透射仪或蓝光成像仪下观察并拍照记录结果。核酸条带会在特定光源激发下发出荧光。

    四、 主要应用

    五、 结果解读

    参考资料:

    1. Sambrook, J., & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual (3rd ed.). Cold Spring Harbor Laboratory Press. (标准实验室手册,详细描述原理与步骤)
    2. Lodish, H., Berk, A., Kaiser, C. A., Krieger, M., Bretscher, A., Ploegh, H., Amon, A., & Scott, M. P. (2016). Molecular Cell Biology (8th ed.). W. H. Freeman. (权威细胞生物学教材,包含基础原理)
    3. National Center for Biotechnology Information (NCBI). Bookshelf: Molecular Biology of the Cell. (在线资源,提供可靠基础知识)
    4. Thermo Fisher Scientific. Agarose Gel Electrophoresis for Nucleic Acids. (知名试剂仪器供应商提供的技术指南,含实用参数)
    5. Green, M. R., & Sambrook, J. (2012). Molecular Cloning: A Laboratory Manual (4th ed.). Cold Spring Harbor Laboratory Press. (更新版经典手册)

    网络扩展资料

    琼脂糖凝胶电泳(Agarose Gel Electrophoresis) 是一种基于核酸分子大小差异进行分离和分析的分子生物学技术。以下是其核心要点:

    1.定义与原理

    2.关键组分

    3.主要应用

    4.注意事项

    补充说明

    该技术因操作简便、成本低,成为分子生物学基础实验之一。如需更详细操作步骤或不同场景的优化方法,可参考、7等来源的完整内容。

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