
瓊脂糖凝膠電泳
Conclusion It is good application prospects to determine the LDH isoenzyme by improvement agarose gel electrophoresis method.
結論改良瓊脂糖凝膠電泳法測定血清LDH同工酶應用前景良好。
Methods PCR reaction systems of RAPD and ISSR were optimized, and the agarose gel electrophoresis results were analyzed into statistic data.
方法優化RAPD及ISSR的PCR反應體系,對産物瓊脂糖凝膠電泳結果進行數據統計。
Methods The length of genetic segment of SCA1, SCA2 and SCA3 in 92 patients with ataxia of unknown origin were detected by polymerase chain reaction (PCR) and agarose gel electrophoresis .
方法應用PCR方法和瓊脂糖凝膠電泳對92例不明原因共濟失調先證患者測定其SCA1、SCA2和SCA3基因片段長度。
The characteristic DNA ladder was found in the agarose gel electrophoresis of the nucleus DNA of tranfsected cells.
細胞核DNA瓊脂糖凝膠電泳呈現凋亡細胞特有的梯狀條帶;
Amplified IPCR products were evaluated by 1.8% agarose gel electrophoresis and ethidium bromide staining.
擴增産物在1-8 % 的瓊脂糖凝膠上進行電泳分離,溴化乙錠染色。
A ladder-like pattern of DNA fragmentation appeared upon agarose gel electrophoresis.
瓊脂糖凝膠電泳可見“梯狀”現象;
DNA agarose gel electrophoresis revealed nuclear fragmentation (DNA ladder).
DNA瓊脂糖凝膠電泳顯示典型的梯狀條帶。
Agarose gel electrophoresis showed evident DNA fragmentation.
DNA瓊脂糖凝膠電泳可見明顯的梯狀條帶;
Methods:Starch-agarose gel electrophoresis is used to observe the interaction of human Hb on rat and mouse Hb.
方法:采用澱粉-瓊脂糖混合凝膠電泳對人及大小鼠血紅蛋白成分分别進行互作實驗,觀察其電泳行為進行判斷。
The result of agarose gel electrophoresis illustrated that it was a better method to separate nucleic acid from cell suspension of histiocyte and blood.
電泳結果表明磁性微球可以很好地從細胞懸液、組織、血液等樣品中分離得到高質量的核酸。
Objective To analyse the clinical feasibility of two electrophoresis procedures of seminal plasma proteins, agarose gel electrophoresis and SDS-agarose gel electrophoresis.
目的探讨瓊脂糖電泳和SDS-瓊脂糖電泳分析精漿蛋白質的臨床可行性。
Methods YAP element was detected by PCR amplification and agarose gel electrophoresis.
方法利用PCR法對此位點進行擴增,經過瓊脂糖凝膠電泳檢測結果。
Agarose gel electrophoresis could be of value in rapid screening and diagnosis of rotavirus infection as a sensitive, specific, and inexpensive test.
可作為一種快速方法用于輪狀病毒感染的篩選和快速診斷。
DNA ladder was detected by agarose gel electrophoresis.
瓊脂糖凝膠電泳檢測DNA梯狀條帶。
Method:The DNA gradent bands were detected by agarose gel electrophoresis.
方法:用瓊脂糖凝膠電泳法觀察DNA的梯狀條帶。
The level of purified superhelix plasmids were measured by 1% agarose gel electrophoresis.
所得純化物以1%瓊脂糖凝膠電泳鑒定超螺旋質粒的含量。
Fluorescence staining and agarose gel electrophoresis were used to study apoptosis.
用熒光染色法和瓊脂糖凝膠電泳觀察細胞凋亡情況。
DNA ladder was showed by agarose gel electrophoresis.
瓊脂糖凝膠電泳測定DNA梯狀帶。
Methods:DNA damage was measured by the loss of fluorescence after DNA binding to ethidium bromide and by the increased DNA mobility in agarose gel electrophoresis.
方法:通過DNA結合溴乙錠後的熒光損失和DNA瓊脂糖凝膠電泳的遷移率來測定DNA的損傷程度。
PCR amplification and agarose gel electrophoresis.
PCR擴增和瓊脂糖凝膠電泳分析。
Amplification products were analyzed by agarose gel electrophoresis.
擴增産物通過瓊脂糖凝膠電泳檢測和分析。
Agarose gel electrophoresis was used to analyze DNA character and the flow cytometry to detect apoptotic rate and cell cycle distribution.
瓊脂糖凝膠電泳分析DNA特征;流式細胞儀檢測細胞凋亡和細胞周期分布;
Methods: Apoptosis was detected with quantitation of DNA fragmentation, agarose gel electrophoresis of DNA ladder, flowcytometric analysis and morphological examination of apoptotic cells.
方法:用DNA電泳、DNA片段定量測定技術、流式細胞術分析及光鏡和電鏡觀察凋亡細胞。
The DNA fragments were detected through agarose gel electrophoresis.
瓊脂糖凝膠電泳檢測DNA的碎片;
Methods:By checking the morphology of apoptotic cells, agarose gel electrophoresis of DNA fragmentation and quantitation of apoptotic cells by means of flow cytometric analysis.
方法:應用細胞形态學檢查,DNA凝膠電泳及流式細胞儀分析檢測。
瓊脂糖凝膠電泳(Agarose Gel Electrophoresis)是分子生物學和生物化學中用于分離、鑒定和純化DNA或RNA片段的一項基礎且關鍵的技術。其核心原理是利用電場驅動帶電生物大分子(主要是核酸)通過瓊脂糖凝膠基質,依據分子大小(長度)進行分離。以下是詳細解釋:
一、 定義與基本原理
二、 關鍵組分與材料
三、 标準操作流程
四、 主要應用
五、 結果解讀
參考資料:
瓊脂糖凝膠電泳(Agarose Gel Electrophoresis) 是一種基于核酸分子大小差異進行分離和分析的分子生物學技術。以下是其核心要點:
該技術因操作簡便、成本低,成為分子生物學基礎實驗之一。如需更詳細操作步驟或不同場景的優化方法,可參考、7等來源的完整内容。
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