
DNA重组技术
In this method, recombinant DNA technology was used to realize data storage, and the sticking model was used to encode information.
该方法以重组DNA技术作为实现DNA数据存储的技术基础,以DNA计算理论研究中的粘贴模型作为信息编码工具。
Biotechnologies for animal nutrition and production are often based on the use of micro-organisms, including those produced through recombinant DNA technology.
在动物营养和生产上所应用的生物技术通常都以微生物的利用为基础,包括那些通过DNA重组技术而生产的微生物。
This process of combining inter-species genes, which is called recombinant DNA technology, does not have the checks and balances that are imposed by nature in traditional breeding.
然而,这种被称作“DNA重组技术”的跨物种的基因合并的过程,不包括自然界中的传统的繁殖过程中所具有的制约与平衡的过程。
Nesiritide is a synthetic form of brain natriuretic peptide using recombinant DNA technology.
奈西立肽是利用重组DNA技术得到的合成型人类脑利钠肽。
As recombinant DNA technology is consummating increasingly, genetic engineering drug is frequently researched and utilized.
随着重组DNA技术的不断完善,基因工程药物的研制和利用日益增多。
The technology is often called modern biotechnology or gene technology, sometimes also recombinant DNA technology or genetic engineering.
该技术通常被称为“现代生物技术”或“基因技术”,有时候也称为“重组脱氧核糖核酸技术”或“遗传工程”。
Before recombinant DNA technology enabled pharmaceutical companies to manufacture human insulin in the 1980s, pig and cow insulin were routinely given to diabetic patients.
在1980年重组DNA技术出现使得药厂制造人类胰岛素出现以前,猪及牛的胰岛素已经成为为糖尿病患者治疗常规使用的药物。
Certain APIs of low molecular weight, such as antibiotics, amino acids, vitamins, and carbohydrates, can also be produced by recombinant DNA technology.
有些低分子量的原料药,如抗生素、氨基酸、维生素和糖类也可以用重组DNA来生产。
In the last few decades, with the development of recombinant DNA technology, metabolic engineering has made tremendous advances.
随着DNA重组技术的日趋成熟,代谢工程的理论和应用已经得到了迅速发展。
Recombinant DNA technology, transgenic technology is also a matter of fact in the molecular level to create the mosaic.
重组DNA技术、转基因技术事实上也都是在制造分子层次上的嵌合体。
Optogenetics combines recombinant DNA technology and optic technology, and is very powerful for cell biology research.
光遗传学结合了重组DNA技术与光学技术,对细胞生物学的研究非常有用。
In 1973, the construction of the first biologically functional bacterial plasmid signaled the beginning of molecular cloning and recombinant DNA technology.
1973年,第一条具有生物学功能的细菌质粒构建成功标志着DNA重组和分子克隆的开始。
This paper has reviewed the recent progress of researches on plant recombinant DNA technology in monocots in 1980s.
本文就国际上八十年代植物重组DNA在单子叶作物上的研究作了综述。
Biochip technology is a great breakthrough of life science after recombinant DNA technology and polymerase chain reaction.
生物芯片技术是继基因工程(重组DNA技术)、聚合酶链式反应(PCR)之后在分子生物学领域中的又一重大突破。
After nearly 40 years of development, recombinant DNA technology has become the foundation of biological research.
经过近40年的发展,基因重组技术已经是研究生物的最基本的技术操作了。
The technology is often called modern biotechnology, orgene technology, sometimes recombinantDNA technology orgenetic engineering.
该技术通常被称为“现代生物技术”或“基因技术”,有时候也称为“重组脱氧核糖核酸技术”或“遗传工程”。
However, this kind of mussel adhesive was confined by ultra-low production, complex technology, and high cost through extraction or recombinant DNA approach.
然而,超低的制备量、复杂的工艺和昂贵的价格限制了通过传统的蛋白提取法和基因工程克隆法制备的贻贝粘合剂的应用。
重组DNA技术(Recombinant DNA Technology)是一种通过人工手段将不同来源的DNA片段进行切割、连接和重组,并将其导入宿主细胞进行复制与表达的分子生物学方法。该技术的核心在于利用限制性内切酶、DNA连接酶等工具酶,将目标基因插入载体(如质粒或病毒DNA),最终实现外源基因在宿主中的功能表达。
基因分离与载体构建
通过限制性内切酶切割供体DNA和载体,再通过DNA连接酶将两者结合。常用载体包括质粒、噬菌体和人工染色体(如BAC)。
宿主转化与筛选
重组DNA通过转化、转染等方式导入宿主细胞(如大肠杆菌或酵母),并利用抗生素抗性标记或荧光报告基因筛选成功重组的细胞。
表达与分析
宿主细胞在适宜条件下扩增重组DNA,并通过蛋白质印迹(Western Blot)或测序验证目标产物,例如人胰岛素、疫苗抗原等。
该技术自1973年由斯坦福大学Cohen和Boyer团队首次实现后,已成为生物医药、农业育种和工业酶生产的基石,并推动了精准医学的发展。
重组DNA技术(Recombinant DNA Technology),又称基因工程或基因拼接技术,是一种通过体外操作将不同来源的DNA片段组合成新遗传物质,并将其导入宿主细胞以实现特定功能的分子生物学技术。以下是详细解释:
如需更完整的流程图示或具体案例,中的技术原理(如)和应用实例(如)。
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