
[細胞] 細胞融合
The cell fusion of fish is an important technology in cell engineering.
人工誘導魚類細胞融合是細胞工程中的一項重要技術。
Instead, the virus required cell activation for replication and mediated cell fusion of T lymphocytes.
相反,該病毒需要細胞激活介導的複制和細胞融合的T淋巴細胞。
Conclusion Expression of B7 molecule in tumor cell can be ameliorated through cell fusion, thereby antigenicity of tumor cell will be raised.
結論通過細胞融合方法可以有效改善腫瘤細胞的B7分子表達,從而提高腫瘤細胞的抗原性。
Objective To provide antigenic cells suitable for cell fusion to preparing lung cancer vaccine.
目的為研制肺癌疫苗提供適合細胞融合的抗原細胞。
In this talk, I shall report our stu***s in transfection and cell fusion and the dynamics during cellular apoptosis introduced by fs beams.
在這個報告裡,我将闡述我們以下的研究:飛秒光緻轉基因、細胞融合、以及細胞在凋亡中的動态過程。
Objective To preparing lung cancer vaccine by cell fusion technique, the lung cancer antigenic cell suitable cell fusion will have to be established.
目的為了通過細胞融合研制肺癌疫苗,需要建立有適合細胞融合的肺癌抗原細胞。
This shows that the PEG-induced fish cell fusion has its specificity in relation to species and tissue.
這表明,PEG誘導的魚類細胞融合具有物種和組織特異性。
Premature chromosome consendesion (PCC) occurred when the cumulus cell nuclei was transferred into enucleated oocyte by directly injection or cell fusion.
将卵丘細胞直接注入卵母細胞或将培養的顆粒細胞與卵母細胞融合,供核細胞都能發生染色質提前凝集(PCC);
The cell fusion instrument consists of high frequency generator, high amplitude pulse generator and sequence control logic unit.
電緻融合儀主要由高頻交流場、高壓窄脈沖發生器以及控制邏輯單元組成。
Compared with chemical or virus fusions the parameters of electrically induced cell fusion is more controllable.
電緻融合比病毒融合、化學融合具有更好可控性。
Chromosome doubling for somatic cells and somatic hybridization via cell fusion are effective methods to create polyploidy.
細胞融合和體細胞染色體加倍是獲得楊樹多倍體種質的有效途徑之一。
The developmental state of ***** cells can be reprogrammed into that of embryonic cells by nuclear transfer, cell fusion or overexpression of specific transcription factors.
成體細胞可以通過核移植、細胞融合或者特定因子導入的方式實現重編程回到多能性狀态。
The electric field-induced cell fusion is a new technique for somatic hybridization since 1980's, and has great application potential in cell genetic engineering.
電場誘導是80年代以來興起的一種細胞融合技術,在細胞遺傳工程實踐中具有很大應用價值。
METHODS: The monoclonal antibody of QPO was produced by cell fusion and identified by Western blotting and the expression of malignant melanoma was stu***d by the immune histochemical technique.
方法:利用細胞融合法制作單克隆抗體,蛋白免疫電泳法檢測單克隆抗體,免疫組化法研究PPO基因在惡性黑色素瘤中的表達情況。
N-linked glycosylation site mutants were got successfully and provide requirement for study the role of N-linked glycosylation on GPs of HTNV in cell fusion.
成功構建了各糖基化位點的突變體,為進一步研究N-連糖基化的缺失對細胞融合的影響提供了必要條件。
Compares the method of cell fusion induced by laser microbeam with induced by virus, PEG, electric field pulse.
比較了微束激光融合法與病毒法,PEG法,電融合法。
The application of the techniques of large_scale cell culture, somatic cell fusion and gene transformation will greatly change the traditional mode of production and breeding of sweet potato.
細胞大規模培養、體細胞融合、基因轉導等技術的研究和應用,可望從根本上改變甘薯傳統的生産和育種模式。
EFF1/AFF1: C. elegans epithelial and anchor cell plasma membrane proteins required for cell fusion.
EFF1/AFF1線蟲上皮細胞和細胞質膜融合錨細胞蛋白用于:。
The works mainly comprise the body cells, cell fusion, nuclear transplantation, cell uptake and the reorganization of chromosome segment.
細胞工程主要包括體細胞融合,核移植,細胞器攝取和染色體片段的重組等。
Cell fusion mediated by tumor viruses, however, may promote the initiation and progression of cancer.
然而,病毒介導的非生理性細胞融合可能促進癌症的發生和發展。
Compared to traditional mutagens, the cell fusion caused by tumor viruses can reasonably explain many phenomena found in some virus-associated cancers.
相對于基因突變等細胞癌變的傳統誘因,病毒的這種緻癌機制能夠更合理地解釋我們在癌症中發現的許多現象。
The ultrastructure of prematurely condensed chromosomes (PCC) in BK cells and the CHO metaphase chromosomes (the PCC inducer) were stu***d with cell fusion technique and SEM.
應用細胞融合技術和掃描電鏡研究了BK(牛腎)細胞早熟凝集染色體(PCC)和誘導PCC的CHO中期染色體的超微結構。
Mitochondrion cavitation, mesenterium insertion and foot cell fusion were found in the model group by an electron microscope.
電鏡結果顯示,腎小球内皮細胞中線粒體多出現低密度或空化,系膜插入,局部足突細胞融合。
The main expressions of CPE consisted in decreased cell diopter, rounding reduced cells, modified particles and formation of multinuclear large cells due to cell fusion.
CPE的主要表現為細胞的折光度降低、細胞圓縮、顆粒變性、細胞融合而形成多核巨細胞。
Somatic cell fusion with this technology in animals has been achieved in mice and voles, mice and chicks, and even mice and people, and many other distant and ultra-wide somatic cell hybridization.
用這種體細胞融合的技術,如今已在動物間實現了小鼠和田鼠,小鼠和小雞,甚至于小鼠和人等許多遠緣和超遠緣的體細胞雜交。
Cytological observation on 5 hybrid cell lines revealed that 2 of them were probably multi-cell fusion products with mean chromosome numbers 167.2 and 198.4 respectively.
對5個雜種細胞系的細胞學觀察表明,其中2個系為多細胞融合産物,其平均染色體數目分别為167.2和198.4;
Aim: To develop a non-infectious cell-cell fusion assay that can be used in a regular biological laboratory for high throughput screening for HIV entry inhibitors.
目的:建立一個基于細胞-細胞融合的HIV進入抑制劑非感染性篩選方法。
Methods From four XP patients, skin fibroblast cell strains were derived and used for the complementation group analysis by autoradiography and cell fusion technique.
方法對着色性幹皮病患者成纖維細胞進行傳代培養、建株,采用放射自顯影和細胞融合技術分析X P細胞互補組。
The electric fusion between the same kind of the naked protoplast of tobacco and barley(normal or yellowy)is stu***d with the DR-1 cell fusion equipment.
利用DR-1型多功能細胞電融合儀分别對煙草和元麥(正常及黃化)的葉肉原生質體進行種内電融合研究。
Including cell fusion technology, cell transplantation, chromosome engineering and tissue culture.
包括細胞融合技術、細胞器移植、染色體工程和組織培養技術。
細胞融合(Cell Fusion)是指兩個或多個獨立的細胞合并形成一個單一細胞的過程,新形成的細胞(稱為合胞體)通常包含來自所有親本細胞的細胞核和細胞質成分。這一現象在生物學中具有重要的生理和實驗意義,可分為自然發生和人工誘導兩大類。
細胞融合的本質是細胞膜的重組。當細胞膜緊密接觸時,其脂質雙層結構發生局部溶解和重組,導緻相鄰細胞的細胞質連通,最終形成多核細胞。這一過程依賴于特定的融合蛋白(如哺乳動物受精過程中的受精素)或病毒融合蛋白(如仙台病毒用于實驗室誘導融合)。
精細胞與卵細胞的融合形成受精卵,是生物有性生殖的基礎。此過程由精細胞表面的Izumo蛋白和卵細胞表面的Juno受體介導,确保物種特異性。
成肌細胞(Myoblasts)通過融合形成多核的肌纖維,以支撐肌肉收縮功能。關鍵調控因子包括肌細胞特異性融合蛋白Myomaker和Myomerger。
哺乳動物胎盤的滋養層細胞融合形成合胞滋養層,促進母胎間的物質交換和免疫屏障建立。
滅活的仙台病毒(Sendai virus)或疱疹病毒可破壞細胞膜脂質結構,促進融合。20世紀60年代首次用于動物細胞雜交實驗。
化學融合劑PEG通過脫水作用使細胞膜緊密黏附并誘發融合,廣泛用于植物原生質體融合和雜交瘤技術。
短脈沖電場使細胞膜暫時穿孔,相鄰細胞在電場作用下接觸并融合。該方法效率高且無化學殘留。
權威參考資料
細胞融合(cell fusion)是細胞遺傳學領域的重要概念,指兩個或多個細胞或原生質體通過自發或人工誘導的方式合并,最終形成單核雜種細胞的過程。以下是詳細解釋:
定義與核心特征
應用領域
相關術語擴展
與細胞融合相關的概念包括核融合(nuclear fusion)、原生質體融合(protoplast fusion)等,常用于描述不同層次的融合現象。
如需進一步了解實驗方法或具體案例,可參考細胞生物學教材或權威研究文獻。
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