
[细胞] 细胞融合
The cell fusion of fish is an important technology in cell engineering.
人工诱导鱼类细胞融合是细胞工程中的一项重要技术。
Instead, the virus required cell activation for replication and mediated cell fusion of T lymphocytes.
相反,该病毒需要细胞激活介导的复制和细胞融合的T淋巴细胞。
Conclusion Expression of B7 molecule in tumor cell can be ameliorated through cell fusion, thereby antigenicity of tumor cell will be raised.
结论通过细胞融合方法可以有效改善肿瘤细胞的B7分子表达,从而提高肿瘤细胞的抗原性。
Objective To provide antigenic cells suitable for cell fusion to preparing lung cancer vaccine.
目的为研制肺癌疫苗提供适合细胞融合的抗原细胞。
In this talk, I shall report our stu***s in transfection and cell fusion and the dynamics during cellular apoptosis introduced by fs beams.
在这个报告里,我将阐述我们以下的研究:飞秒光致转基因、细胞融合、以及细胞在凋亡中的动态过程。
Objective To preparing lung cancer vaccine by cell fusion technique, the lung cancer antigenic cell suitable cell fusion will have to be established.
目的为了通过细胞融合研制肺癌疫苗,需要建立有适合细胞融合的肺癌抗原细胞。
This shows that the PEG-induced fish cell fusion has its specificity in relation to species and tissue.
这表明,PEG诱导的鱼类细胞融合具有物种和组织特异性。
Premature chromosome consendesion (PCC) occurred when the cumulus cell nuclei was transferred into enucleated oocyte by directly injection or cell fusion.
将卵丘细胞直接注入卵母细胞或将培养的颗粒细胞与卵母细胞融合,供核细胞都能发生染色质提前凝集(PCC);
The cell fusion instrument consists of high frequency generator, high amplitude pulse generator and sequence control logic unit.
电致融合仪主要由高频交流场、高压窄脉冲发生器以及控制逻辑单元组成。
Compared with chemical or virus fusions the parameters of electrically induced cell fusion is more controllable.
电致融合比病毒融合、化学融合具有更好可控性。
Chromosome doubling for somatic cells and somatic hybridization via cell fusion are effective methods to create polyploidy.
细胞融合和体细胞染色体加倍是获得杨树多倍体种质的有效途径之一。
The developmental state of ***** cells can be reprogrammed into that of embryonic cells by nuclear transfer, cell fusion or overexpression of specific transcription factors.
成体细胞可以通过核移植、细胞融合或者特定因子导入的方式实现重编程回到多能性状态。
The electric field-induced cell fusion is a new technique for somatic hybridization since 1980's, and has great application potential in cell genetic engineering.
电场诱导是80年代以来兴起的一种细胞融合技术,在细胞遗传工程实践中具有很大应用价值。
METHODS: The monoclonal antibody of QPO was produced by cell fusion and identified by Western blotting and the expression of malignant melanoma was stu***d by the immune histochemical technique.
方法:利用细胞融合法制作单克隆抗体,蛋白免疫电泳法检测单克隆抗体,免疫组化法研究PPO基因在恶性黑色素瘤中的表达情况。
N-linked glycosylation site mutants were got successfully and provide requirement for study the role of N-linked glycosylation on GPs of HTNV in cell fusion.
成功构建了各糖基化位点的突变体,为进一步研究N-连糖基化的缺失对细胞融合的影响提供了必要条件。
Compares the method of cell fusion induced by laser microbeam with induced by virus, PEG, electric field pulse.
比较了微束激光融合法与病毒法,PEG法,电融合法。
The application of the techniques of large_scale cell culture, somatic cell fusion and gene transformation will greatly change the traditional mode of production and breeding of sweet potato.
细胞大规模培养、体细胞融合、基因转导等技术的研究和应用,可望从根本上改变甘薯传统的生产和育种模式。
EFF1/AFF1: C. elegans epithelial and anchor cell plasma membrane proteins required for cell fusion.
EFF1/AFF1线虫上皮细胞和细胞质膜融合锚细胞蛋白用于:。
The works mainly comprise the body cells, cell fusion, nuclear transplantation, cell uptake and the reorganization of chromosome segment.
细胞工程主要包括体细胞融合,核移植,细胞器摄取和染色体片段的重组等。
Cell fusion mediated by tumor viruses, however, may promote the initiation and progression of cancer.
然而,病毒介导的非生理性细胞融合可能促进癌症的发生和发展。
Compared to traditional mutagens, the cell fusion caused by tumor viruses can reasonably explain many phenomena found in some virus-associated cancers.
相对于基因突变等细胞癌变的传统诱因,病毒的这种致癌机制能够更合理地解释我们在癌症中发现的许多现象。
The ultrastructure of prematurely condensed chromosomes (PCC) in BK cells and the CHO metaphase chromosomes (the PCC inducer) were stu***d with cell fusion technique and SEM.
应用细胞融合技术和扫描电镜研究了BK(牛肾)细胞早熟凝集染色体(PCC)和诱导PCC的CHO中期染色体的超微结构。
Mitochondrion cavitation, mesenterium insertion and foot cell fusion were found in the model group by an electron microscope.
电镜结果显示,肾小球内皮细胞中线粒体多出现低密度或空化,系膜插入,局部足突细胞融合。
The main expressions of CPE consisted in decreased cell diopter, rounding reduced cells, modified particles and formation of multinuclear large cells due to cell fusion.
CPE的主要表现为细胞的折光度降低、细胞圆缩、颗粒变性、细胞融合而形成多核巨细胞。
Somatic cell fusion with this technology in animals has been achieved in mice and voles, mice and chicks, and even mice and people, and many other distant and ultra-wide somatic cell hybridization.
用这种体细胞融合的技术,如今已在动物间实现了小鼠和田鼠,小鼠和小鸡,甚至于小鼠和人等许多远缘和超远缘的体细胞杂交。
Cytological observation on 5 hybrid cell lines revealed that 2 of them were probably multi-cell fusion products with mean chromosome numbers 167.2 and 198.4 respectively.
对5个杂种细胞系的细胞学观察表明,其中2个系为多细胞融合产物,其平均染色体数目分别为167.2和198.4;
Aim: To develop a non-infectious cell-cell fusion assay that can be used in a regular biological laboratory for high throughput screening for HIV entry inhibitors.
目的:建立一个基于细胞-细胞融合的HIV进入抑制剂非感染性筛选方法。
Methods From four XP patients, skin fibroblast cell strains were derived and used for the complementation group analysis by autoradiography and cell fusion technique.
方法对着色性干皮病患者成纤维细胞进行传代培养、建株,采用放射自显影和细胞融合技术分析X P细胞互补组。
The electric fusion between the same kind of the naked protoplast of tobacco and barley(normal or yellowy)is stu***d with the DR-1 cell fusion equipment.
利用DR-1型多功能细胞电融合仪分别对烟草和元麦(正常及黄化)的叶肉原生质体进行种内电融合研究。
Including cell fusion technology, cell transplantation, chromosome engineering and tissue culture.
包括细胞融合技术、细胞器移植、染色体工程和组织培养技术。
细胞融合(Cell Fusion)是指两个或多个独立的细胞合并形成一个单一细胞的过程,新形成的细胞(称为合胞体)通常包含来自所有亲本细胞的细胞核和细胞质成分。这一现象在生物学中具有重要的生理和实验意义,可分为自然发生和人工诱导两大类。
细胞融合的本质是细胞膜的重组。当细胞膜紧密接触时,其脂质双层结构发生局部溶解和重组,导致相邻细胞的细胞质连通,最终形成多核细胞。这一过程依赖于特定的融合蛋白(如哺乳动物受精过程中的受精素)或病毒融合蛋白(如仙台病毒用于实验室诱导融合)。
精细胞与卵细胞的融合形成受精卵,是生物有性生殖的基础。此过程由精细胞表面的Izumo蛋白和卵细胞表面的Juno受体介导,确保物种特异性。
成肌细胞(Myoblasts)通过融合形成多核的肌纤维,以支撑肌肉收缩功能。关键调控因子包括肌细胞特异性融合蛋白Myomaker和Myomerger。
哺乳动物胎盘的滋养层细胞融合形成合胞滋养层,促进母胎间的物质交换和免疫屏障建立。
灭活的仙台病毒(Sendai virus)或疱疹病毒可破坏细胞膜脂质结构,促进融合。20世纪60年代首次用于动物细胞杂交实验。
化学融合剂PEG通过脱水作用使细胞膜紧密黏附并诱发融合,广泛用于植物原生质体融合和杂交瘤技术。
短脉冲电场使细胞膜暂时穿孔,相邻细胞在电场作用下接触并融合。该方法效率高且无化学残留。
权威参考资料
细胞融合(cell fusion)是细胞遗传学领域的重要概念,指两个或多个细胞或原生质体通过自发或人工诱导的方式合并,最终形成单核杂种细胞的过程。以下是详细解释:
定义与核心特征
应用领域
相关术语扩展
与细胞融合相关的概念包括核融合(nuclear fusion)、原生质体融合(protoplast fusion)等,常用于描述不同层次的融合现象。
如需进一步了解实验方法或具体案例,可参考细胞生物学教材或权威研究文献。
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