[生化] 辣根*********物酶
Objective:To clone and express the horseradish peroxidase isozyme C3(HRPC3) gene.
目的：克隆與表達辣根*********物酶同功酶C3（HRPC3）基因。
Based on the inhibition of cyanide to the horseradish peroxidase(HRP), the HRP electrode has been used in the determination of cyanide in water.
基于*********對辣根*********物酶（HRP）的抑制作用，将辣根*********物酶電極用于水中的微量*********的測定。
Unilateral injection of horseradish peroxidase into the muscle resulted in bilateral labelling of cell in the ventromedial region of the caudal trigeminal nucleus.
将辣根*********物酶注入單側肌肉内，在三叉神經運動核尾側的腹内側部引起了雙側的标記細胞。
The Electrochemical behavior of the product, 2,3 diaminophenazine (DAP), of the enzymatic catalytic reaction by horseradish peroxidase was stu***d by various electrochemical methods.
用多種電化學分析方法研究了辣根*********物酶催化反應産物2 ，3-*********吩嗪（DAP）的電化學行為。
This Cell Signaling Technology (CST) antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.
CST公司的該抗體通過其氨基結合了辣根*********物酶（HRP）的碳水化合物基團。
Afferent connections of the oaudoputamen in the rat were stu***d by axonal retrograde transport method using horseradish peroxidase (HRP).
應用辣根*********物酶（HRP）的逆行傳遞技術研究了大鼠尾殼複合體的傳入性聯繫。
After the horseradish peroxidase (HRP) was treated with different strengths of electric field, the HRP and activity index were assayed by ultraviolet-visible spectra.
用不同電場強度處理辣根*********物酶，測試電場處理前後的紫外-可見光譜和酶的活力指數。
Horseradish peroxidase was used to label the regenerative axons of peroneal nerves 4 or 9 weeks after epineurial or Perineurial suture or nerve grafting in sciatic nerves in rats.
大鼠坐骨神經外膜縫合、束膜縫合或神經移植後4周或9周，應用辣根*********物酶逆行标記腓總神經中再生軸突的胞體。
The distribution of lectin binding in gastrointestinal tract of human, rat and mouse was investigated using horseradish peroxidase labeled lectins-WGA, SBA, and PNA staining method.
用三種辣根*********物酶标記的凝集素—WGA、SBA和PNA觀察人及大小鼠的消化管各段中凝集素結合部位的分布。
Transepithelial electrical resistance(TER)was detected and the permeability was measured with fluorescein sodium(FS)and horseradish peroxidase(HRP).
行跨膜電阻測定并用熒光素鈉和辣根*********物酶（HRP）進行通透性檢測。
The reaction between the TMB substrate and a peroxidase, typically horseradish peroxidase (HRP), produces a measureable color change that correlates with analyte level.
TMB與*********物酶，典型的是辣根*********物酶（HRP）之間的反應可以産生可測量的與分析物相關顔色變化。
Insulin was labeled with horseradish peroxidase by sodium periodate oxidation method.
用*********鈉氧化法将辣根*********物酶标記于胰島素。
Thyroxine (T_4) was coupled with horseradish Peroxidase(HRP) by a two-step linking method of glutaraldehyde.
采用*********二步法使辣根*********物酶（HRP）與半抗原T_4相偶聯。
Lignin and phenol was copolymerized with horseradish peroxidase as catalyst in reversed micelles, and the copolymer was analyzed by infra red and differential scanning calorimetry.
在反相微乳液系統中，用辣根*********物酶進行了酶促木質素與酚共聚反應，并進行了紅外光譜分析及差示掃描量熱分析。
The monoclonal antibody against IL-2 was labelled with horseradish peroxidase.
用辣根*********物酶标記IL-2單克隆抗體。
A double substrates enzyme reaction, which horseradish peroxidase (HRP) catalytic oxide of o -phenylenediamine (OPD) by H2O2, were stu***d by thermokinetic method.
利用微量熱方法研究了辣根*********物酶（HRP）催化H_2O_2氧化************（OPD）的反應。
The distribution of lectin receptor in liver and pancreas of rats and mice was investigated using WGA, SBA, PNA labeled with horseradish peroxidase.
本文用辣根*********物酶标記的麥芽凝集素、大豆凝集素及花生凝集素來研究大、小鼠肝、胰中凝集素受體的分布。
A voltammetric enzyme - linked immunoassay based on a new system of o- tilidine (OT) - H2O2 - horseradish peroxidase (HRP) has firstly been developed.
本文首次提出鄰聯*********（OT）-H_2O_2-辣根*********物酶（HRP）伏安酶聯免疫分析新體系。
A pair of redox peaks attributed to the direct redox reaction of horseradish peroxidase in the phosphate buffer solution was observed.
結果顯示：在磷酸緩沖溶液中，辣根*********物酶在钛酸納米管修飾的玻碳電極上呈現出一對良好的氧化還原峰。
Distribution of LH cells in the anterior pituitary of the female rats were stu***d using the horseradish peroxidase immunohistochemical method.
應用辣根*********物酶标記抗體技術，對正常和閹割成年雌性大白鼠垂體LH細胞的分布進行了研究。
Horseradish peroxidase was immobilized by bovine serum albumin and glutaraldehyde on the surface of the glassy carbon electrode, which was modified with hydroquinone.
用牛血清白蛋白和*********把辣根*********物酶固定在************修飾的電極上，制成*********氫傳感器。
AGE-BSA of different concentration was applied to BREC in different time. And Horseradish peroxidase (HRP) engulfed by BREC was surveyed.
檢測不同濃度AGE-BSA在不同時間對BREC内吞辣根*********物酶（HRP）情況。
Anterior pagoda (AP) neurons of leeches were marked by intracellular injection of horseradish peroxidase (HRP).
用胞内注射辣根*********物酶（HRP）的方法标記中華寬體金線蛭AP神經元。
Taking horseradish peroxidase (HRP) as an enzyme model, this presented platform has been successfully exploited to develop a novel electrochemical enzyme sensor for the detection of H2O2.
以固定辣根*********物酶（HRP）為例，發展了一種新的電化學酶傳感器用于H2O2的測定。
Anti-biotin (D5A7) Rabbit mAb is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.
抗生物素（D5A7）兔源抗體（HRP标記）可以與辣根*********物酶通過氣氨基基團與碳水化合物基團結合。
The development of antibody—producing immunocytes was examined in the popliteal lymph nodes of the guinea pig during their primary response to horseradish peroxidase (HRP).
本文以辣根*********物酶（HRP）與福氏完全佐劑混合成的乳液對雄性豚鼠作一次免疫，追蹤腘淋巴結中抗體形成細胞的分化途徑和變化。
辣根過氧化物酶（Horseradish Peroxidase, HRP） 是一種廣泛存在于辣根（Armoracia rusticana）等植物中的氧化還原酶，屬于過氧化物酶超家族。其核心功能是催化過氧化物（如過氧化氫，H₂O₂）對多種有機和無機底物的氧化反應，同時伴隨發色團或熒光團的顯色變化。以下是其詳細解釋：
酶學特性
HRP是一種糖蛋白，分子量約44 kDa，含有一個高自旋鐵（Ⅲ）原卟啉IX（血紅素）輔基作為活性中心。其催化機制依賴于血紅素鐵離子的價态變化（Fe³⁺ ↔ Fe⁴⁺），通過中間體化合物I和II實現電子轉移。典型反應式為：
$$ ce{ROOR' + 2e^- + 2H+ -> ROH + R'OH} $$
其中ROOR'代表過氧化物底物（如H₂O₂）。
底物特異性
HRP可催化多種供氫體（如鄰苯二胺、四甲基聯苯胺）的氧化，産生有色或熒光産物。例如：
免疫檢測技術
HRP是酶聯免疫吸附試驗（ELISA）和免疫印迹（Western Blot）的關鍵标記酶。其偶聯于抗體後，可通過底物顯色定量檢測抗原，靈敏度達皮摩爾級别。例如，COVID-19抗體檢測試劑盒常采用HRP-抗體複合物。
分子生物學探針
在原位雜交和熒光原位雜交（FISH）中，HRP标記的核酸探針可催化酪胺信號放大（TSA），實現低豐度靶标的可視化檢測。
生物傳感器與診斷
HRP用于構建電化學生物傳感器，通過檢測H₂O₂濃度間接定量葡萄糖、膽固醇等代謝物。其高催化效率（k_cat≈10⁷ s⁻¹）使其成為理想的生物标志物放大器。
HRP在辣根中作為防禦蛋白，通過氧化酚類物質生成醌類毒素抵禦病原體侵染。其天然豐度高（占可溶性蛋白的~1%），且具有耐熱性（最適溫度40-50℃）和pH穩定性（pH 5-9），適合工業化純化與應用。
酶學機制與結構
應用技術
植物生理學背景
horseradish peroxidase（HRP） 是一種重要的酶類，以下為詳細解釋：
定義與來源
HRP是從辣根（Amoracia rusticana）根部提取的過氧化物酶，屬于鐵原卟啉酶類，由酶蛋白和鐵卟啉輔基結合而成。其英文縮寫為HRP（Horseradish Peroxidase）。
結構與特性
應用領域
主要用于免疫組化中标記抗原與抗體，也用于生物化學實驗的催化反應（如ELISA檢測）。此外，可通過化學修飾（如炔基化）擴展其功能。
其他補充
HRP的輔基為棕色的鐵卟啉，而酶蛋白部分呈無色，整體為糖蛋白結構。
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