柱層析法；[分化] 柱色譜法
Determining the content of total saponin by column chromatography.
柱層析法分離測定楤木總苷含量；
Methods: (1)To separate stilbene glycoside by using silica gel column chromatography with gra***nt elution consisting of ethyl acetate and petroleum ether.
方法：（1）以矽膠柱色譜法，************-石油醚梯度洗脫，自何首烏中提取分離************苷單體成分。
The method of extracting the auxin in seaweeds with organic solvent, separating and purifying the auxin by ether extraction method and column chromatography has been established.
确立了以有機溶劑提取海藻中的生長素，以乙醚萃取法和柱層析法對生長素進行分離純化的方法。
In this paper it is reported that using TLC PC and PE are first separated by column chromatography further.
本文報道以薄層層析為先導，用柱層析法分離磷脂酰膽堿（PC）和磷脂酰*********（PE）。
Solanesol was isolated and purified from the extracts of potato leaves by column chromatography, and RP-HPLC method was established for determining the content of solanesol.
通過柱色譜法從馬鈴薯葉的提取物中分離提純了茄尼醇，并以RP-HPLC法測定了茄尼醇的質量分數。
Methods: The compounds were isolated by silica gel column chromatography, and their structures were elucidated by spectral methods.
方法：利用矽膠柱色譜法對化合物進行分離，運用波譜方法确定結構。
This research was manage to separate them by dry column chromatography.
文中采用幹柱層析法對其進行分離。
Silica gel column chromatography and solvent extraction were used to separate grease and abradant in a kind of excellent polishing paste for plastics, respectively.
分别采用矽膠柱色譜法和溶劑抽提，分離優質塑料抛光膏中的油脂和磨料部分。
In this paper, we extracted the antioxidative substance from Alpinia oxyphylla Miquel by using column chromatography.
探讨了幹柱層析法分離益智渣及益智乙醇提取物的抗氧化成分。
The inhibin plasmid DNA was extracted and purified by alkaline lysis method and DEAE-fiber column chromatography and its concn.
采用堿裂解法與DEAE-纖維柱層析法提取與純化抑制素質粒DNA，用分光光度法測定其濃度和純度。
Several byproducts in alkylbenzene sulfonic acid were separated through column chromatography and identified by mass spectrum(MS).
采用柱層析法分離出了烷基苯磺酸中的雜質，并采用質譜法對這些雜質進行了定性。
METHOD Curcumin was extracted from turmeric by ethanol, and separated from others with the column chromatography.
方法用醇提法從姜黃中提取姜黃素，用柱色譜法把姜黃素從醇提物中與其它雜質分離。
Method: The compounds were extracted with solvents, isolated by various column chromatography and identified by spectroscopic methods.
方法：溶劑提取及各種柱色譜法分離，光譜技術鑒定結構。
The effective and active components in bamboo leaves were separated using gra***nt elution and column chromatography.
采用梯度洗脫及柱色譜法對竹葉中的有效活性成分重點為黃酮進行了分離研究、提純。
The crude products were purified by neutral alumina column Chromatography with dichloromethane containing pyridine as eluent.
用中性氧化鋁柱層析法對粗産物進行分離提純，用含一定量吡啶的************溶液作為洗脫劑。
The Ginsenoside CompundK from enzyme reaction was isolated by silica gel column chromatography.
研究了矽膠柱層析法對酶反應産物皂苷C K粗品進行的分離純化。
Specific solvent separation, acid and alkali treatment, enzymatic hydrolysis and column chromatography are the main methods to be used in the refinement of curcumin.
精制方法主要包括專一性溶劑法、酸堿法、酶水解法和柱層析法等。
METHODS Silica, polyamide and ODS column chromatography were used.
方法采用矽膠、聚酰胺和ODS柱層析法。
METHODS Recrystallization and column chromatography were used to get purer hyodeoxycholic acid and some other components.
方法運用重結晶和柱層析法進行分離和純化。
Dynamic and static ion exchange method, and silica gel column chromatography were used to separate ion and nonion components in synthetic fiber oil agent of unknown composition, respectively.
本文分别采用動态及靜态離子交換法和矽膠柱色譜法，分離未知組成紡織油劑中的離子組分和非離子組分；
Methods:The constituents were isolated and purified by using column chromatography with polyamide and appraised by thin-layer chromatography and Ultraviolet spectrometry.
方法：先利用聚酰胺柱層析法分離，再用薄層色譜法和紫外光譜法進行鑒定，并以蘆丁為标準品，采用紫外分光光度法進行含量測定。
Dynamic and static ion exchange methods, and silica gel column chromatography were used to separate ion and nonion components in a synthetic fiber oil agent of unknown composition, respectively.
分别采用動态及靜态離子交換法和矽膠柱色譜法，分離未知組成合成纖維油劑中的離子組分和非離子組分；
METHODS ? The isolation and purification was performed by silica gel column chromatography and the structures were identified by spectral analysis.
用矽膠柱層析法分離純化，用光譜法進行結構鑒定。
Phosphatidylcholine of high purity(PC, content 92.80%) was prepared from market soybean power phospholipids(PC content 14.05%) by using solvent extraction and column chromatography.
采用溶劑提取和柱色譜法分離純化市售大豆粉末磷脂（卵磷脂含量14.05%），得到高純度的卵磷脂産品（純度92.80%）。
This paper is to discuss the feasibility of refining taurine in the extract of Meretrix meretrix by column chromatography and determination of taurine by spectrophotometry.
作者主要探索用離子交換樹脂柱析法提純文蛤提取液中牛磺酸及用吸收光譜法測定牛磺酸的可行性。
Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
用離子交換柱層析法分離，用反相高效液相色譜法進一步純化，經聚************凝膠電泳分析确定相對分子質量。
柱色譜法（Column Chromatography）是一種基于物質在固定相和流動相之間分配差異實現分離的實驗室技術。其核心原理是利用不同化合物在固定相（如矽膠、氧化鋁）與流動相（溶劑）中的吸附或分配能力差異，通過連續洗脫使混合物中的成分分層析出。
該方法通常分為三個步驟：
在生物化學領域，柱色譜常用于蛋白質純化（如親和色譜）；在制藥工業中，則廣泛應用于天然産物有效成分的提取分離（參考自《Journal of Chromatography A》期刊研究）。現代高效液相色譜（HPLC）作為其衍生技術，已實現更高精度的分析檢測（美國化學會ACS Publications數據）。
（注：實際引用應替換為真實文獻鍊接，此處因搜索限制保留标注格式）
柱色譜法（Column Chromatography）是一種基于物質在固定相和流動相之間分配差異的分離技術，廣泛應用于化學、生物化學和制藥領域。以下是詳細解釋：
1. 基本原理
通過不同成分在固定相（如矽膠、氧化鋁）和流動相（溶劑）中的吸附/解吸能力差異實現分離。極性強的物質更易吸附在固定相上，移動速度慢；極性弱的物質則隨流動相快速洗脫。
2. 操作步驟
3. 主要應用
4. 類型與優化
注意事項：需避免柱内氣泡或裂縫，控制流速防止拖尾現象。實驗室中常與旋轉蒸發儀聯用，用于濃縮洗脫後的目标組分。
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