restriction endonuclease是什么意思，restriction endonuclease的意思翻译、用法、同义词、例句

限制酶，限制性内切核酸酶

The recombinant plasmid PET- 11D-ospC was identified with restriction endonuclease analysis and sequencing.

采用酶切分析及序列测定等方法鉴定重组质粒的正确性。

In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.

本研究利用多种组合的酶切分析，绘制了较为详细的酶切图谱。

Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.

结果经PCR方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。

Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;

并对发现突变的基因进行分析。方法 应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；

Restriction enzyme:Protein (more specifically, an endonuclease) produced by bacteria that cleaves DNA at specific sites along its length.

限制性内切：由细菌产生的一种蛋白质，能在特定的地方切断去氧核糖核酸分子。 收藏。

Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the agar gel electrophoresis.

最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。

Methods:DNA sequencing and restriction endonuclease reaction was used.

方法：采用DNA测序及限制性酶切反应方法。

Finally, the cloned B antigen gene was identified by restriction endonuclease analysis, DIG probe hybridization and DNA sequencing.

通过内切酶分析、*********（DIG）—探针原位杂交、DNA序列测定，确认克隆的B抗原基因正确。

Janpanese encephalitis virus; recombinant plasmid; DNA sequencing; restriction endonuclease analysis.

流行性乙型脑炎病毒；重组质粒；DNA测序；限制性内切酶分析。

CP4-EPSPS gene was amplified from 13 samples, but all of them were proved to be false positives in restriction endonuclease digestion analysis of PCR products.

有13个样品在CP4-EPSPS基因检测中虽扩增出类似阳性对照的条带，但对PCR产物的进一步酶切鉴定表明，所有扩增结果为假阳性。

Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful.

结果酶切及测序显示对OSCP基因有义突变部分的纠正获得成功。

Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.

方法用盐提取法提取人群中白细胞的DNA，以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。

Secondly, the preamplified DNA fragments were digested by a restriction endonuclease to form sticky ends, which were then ligated to a designed DNA adapter by ligase.

然后用限制性内切酶将其消化成短片段，在连接酶的作用下与设计的DNA适配器相连；

The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.

建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。

Then, we analyzed the specificity of PCR primers through digesting PCR products with restriction endonuclease.

然后对扩增产物进行限制性酶切反应；

After a restriction endonuclease cleavage, two expected smaller fragments were observed.

经限制性内切酶反应，获得两个预期大小的片段。

Result The recombinant was correctly constructed and restriction endonuclease analysis and PCR amplification.

结果 经酶切鉴定及基因测序证实重组三突变型穿梭质粒构建成功。

|restriction enzyme;限制酶，限制性内切核酸酶